GEO DataSets

(Submitter supplied) Sexual reproduction (meiosis and syngamy) is the major form of reproduction in diploid Boechera species, but most species hybrids reproduce by apomixis (unreduced gametophyte formation followed by parthenogenesis of the unreduced egg). In this study, we used Arabidopsis microarrays to detail global programs of gene expression underlying sexual and apomictic modes of reproduction.

Organism:

Boechera stricta; Boechera microphylla; Arabidopsis thaliana; Boechera lignifera; Boechera formosa x Boechera sp. JC-2020

Type:

Expression profiling by array

Platform:

GPL198

20 Samples

Download data: CEL, TXT

(Submitter supplied) Apomixis differs from sexual reproduction only in three major aspects: While the sexual megaspore mother cell undergoes meiosis, the apomictic initial cell omits or aborts meiosis (apomeiosis); the unreduced egg cell of apomicts forms an embryo without fertilization (parthenogenesis), and formation of functional endosperm requires specific developmental adaptations. Currently, our knowledge about the gene regulatory programs underlying apomixis is scarce. more...

Organism:

Boechera gunnisoniana; Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL16112

7 Samples

Download data: CEL

(Submitter supplied) Apomixis defines a number of reproductive strategies, which, unlike sexual reproduction, permit the inheritance of the maternal genome over generations without genetic recombination events. The key biological features of apomixis are the failure of meiosis (i.e., apomeiosis), the differentiation of unreduced embryo sacs and eggs, and their autonomous development in functional embryos through parthenogenesis and the formation of viable endosperm either via fertilization-independent means or following fertilization with a sperm cell (Koltunow and Grossniklaus 2003). more...

Organism:

Hypericum perforatum

Type:

Expression profiling by array

Platform:

GPL22204

12 Samples

Download data: TXT

(Submitter supplied) The absence of meiosis and sex are expected to lead to mutation accumulation in asexual (apomictic) plants. We have performed a double-validated analysis of copy number variation (CNV) on 10 biological replicates each of diploid sexual and diploid apomictic Boechera using a high-density (>700K) custom microarray, in order to compare mutation accumulation in the form of CNV between the transcribing regions of their genomes. more...

Organism:

Boechera retrofracta; Boechera crandallii; Boechera polyantha; Boechera stricta; Boechera holboellii; Boechera schistacea; Boechera retrofracta x Boechera stricta; Boechera duchesnensis; Boechera lignifera; Boechera divaricarpa

Type:

Genome variation profiling by genome tiling array

Platform:

GPL17827

40 Samples

Download data: GFF

(Submitter supplied) Next-generation sequencing was employed to compare transcriptomic profiling of ovules at and prior to the appearance of nucellar embryony initial cells. To exclude the unwanted genetic background noise and variations among years, two biological replicates for each sample were set by sampling in two consecutive years from two pairs of polyembryonic and monoembryonic cultivars, i.e. one pair of mandarin and the other of grapefruit/pummelo. more...

Organism:

Citrus reticulata; Citrus x clementina; Citrus maxima; Citrus x paradisi

Type:

Expression profiling by high throughput sequencing

4 related Platforms

16 Samples

Download data: XLSX

(Submitter supplied) To identify miRNAs related nucellar embryo intiation (NEI) in citrus, we performed small RNA sequencing in ovules of two pairs of poly- and mono-embryonic cultivars right prior to and at NEI stages. Nearly 150 miRNAs including ~90 conserved and ~60 novel miRNAs were identified in ovules of each cultivar pair.

Organism:

Citrus maxima; Citrus x clementina; Citrus x paradisi; Citrus reticulata

Type:

Non-coding RNA profiling by high throughput sequencing

4 related Platforms

16 Samples

Download data: TXT

(Submitter supplied) Maize plants defective for the AGO104 protein show defects in chromatin condensation during meiosis, and subsequent failure to segregate chromosomes. AGO104 is a member of the ARGONAUTE family of proteins. AGO104 accumulates specifically in somatic cells surrounding the female meiocyte, suggesting a mobile signal rather than cell-autonomous control. AGO104 is necessary for non-CG methylation of centromeric and knob repeat DNA. more...

Organism:

Zea mays

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9141

2 Samples

Download data

(Submitter supplied) Hypericum perforatum L. (2n=4x=32) is an attractive model system for the study of aposporous apomixis. The earliest phenotypic features of aposporous apomixis in this species are the mitotic formation of unreduced embryo sacs from a somatic cell of the ovule nucellus and the avoidance of meiosis. In this research we addressed gene expression variation in sexual and apomictic plants, by focusing on the ovule nucellus,  which is the cellular domain primarily involved into the differentiation of meiocyte precursors and aposporous embryo sacs. more...

Organism:

Hypericum perforatum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21906

6 Samples

Download data: TXT

(Submitter supplied) Angiosperms possess a characteristic life cycle with an alternation of sporophyte and gametophyte generations, which happens in plant organs like pistils. Rice pistils contain ovules and receive pollen for successful fertilization to produce grains. The cellular expression profile in rice pistils is largely unknown. Here we show a cell atlas of rice pistils before fertilization by droplet-based single-nucleus RNA sequencing. more...

Organism:

Oryza sativa Japonica Group

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27860

11 Samples

Download data: MTX, TSV

(Submitter supplied) Angiosperms possess a characteristic life cycle with an alternation of sporophyte and gametophyte generations, which happens in plant organs like pistils. Rice pistils contain ovules and receive pollen for successful fertilization to produce grains. The cellular expression profile in rice pistils is largely unknown. Here we show a cell atlas of rice pistils before fertilization by droplet-based single-nucleus RNA sequencing. more...

Organism:

Oryza sativa Japonica Group

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27860

3 Samples

Download data: MTX, TSV

(Submitter supplied) Angiosperms possess a characteristic life cycle with an alternation of sporophyte and gametophyte generations, which happens in plant organs like pistils. Rice pistils contain ovules and receive pollen for successful fertilization to produce grains. The cellular expression profile in rice pistils is largely unknown. Here we show a cell atlas of rice pistils before fertilization by droplet-based single-nucleus RNA sequencing. more...

Organism:

Oryza sativa Japonica Group

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27860

8 Samples

Download data: TXT