GEO DataSets

(Submitter supplied) To test if HIF pathway is regulated by lipids in zebrafish larvae, we have employed whole genome microarray expression profiling as a discovery platform to identify induced genes upon lalistat or U18666A treatment. Lalistat is a specific inhibitor for lysosomal acid lipase (LAL), which is required for break down triglyceride or cholesterol ester to release free fatty acids. U18666A is a specific inhibitor for NPC, which is required for export free cholesterol oout of lysosomes.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL14688

9 Samples

Download data: TXT

(Submitter supplied) To test if HIF pathway is regulated by lipids in zebrafish larvae, we have employed whole genome microarray expression profiling as a discovery platform to identify induced genes upon lalistat treatment. Lalistat is a specific inhibitor for lysosomal acid lipase (LAL), which is required for break down triglyceride or cholesterol ester to release free fatty acids.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14688

4 Samples

Download data: TXT

(Submitter supplied) To assay differently expressed genes in PDAC cells Pa03c cultured in regular 10% FBS medium or lipoprotein depleted 10% LPDS medium

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10904

2 Samples

Download data: IDAT, TXT

(Submitter supplied) Mitochondria fulfill vital metabolic functions and act as crucial cellular signaling hubs integrating their metabolic status into the cellular context. Here, we show that defective cardiolipin-remodeling, upon loss of the cardiolipin acyl transferase Tafazzin, mutes HIF-1a signaling in hypoxia. Tafazzin-deficiency does not affect posttranslational HIF-1a regulation but rather HIF-1a gene-expression, a dysfunction recapitulated in iPSCs-derived cardiomyocytes from Barth Syndrome patients with Tafazzin-deficiency. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

12 Samples

Download data: TXT

(Submitter supplied) Orogastral infection of mice with Yersinia enterocolitical leads to HIF-1 alpha activation.To elucidate whether this HIF-1 alpha activation also results in a HIF-1 dependent gene programming, the transcriptomes from Peyers Patches of uninfected and Yersinia enterocolitica infected mice were analyzed by means of of microarray analyses using Affymetrix GeneChip probe arrays (MG-U74Av2). In total, 288 genes were differentially regulated three day after infection in PP compared with the expression of uninfected control mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

3 Samples

Download data: CEL, CHP

(Submitter supplied) The response of cells to hypoxia is characterised by co-ordinated regulation of many genes. Studies of the regulation of the expression of many of these genes by oxygen has implicated a role for the heterodimeric transcription factor hypoxia inducible factor (HIF). The mechanism of oxygen sensing which controls this heterodimeric factor is via oxygen dependent prolyl and asparaginyl hydroxylation by specific 2-oxoglutarate dependent dioxygenases (PHD1, PHD2, PHD3 and FIH-1). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2758 GDS2759 GDS2760 GDS2761

Platforms:

GPL2507 GPL96 GPL570

39 Samples

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Analysis of hypoxic MCF-7 breast cancer cells depleted for hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, or both HIF alpha subunits. Results provide insight into the extent of the role of HIF-1alpha and HIF-2alpha in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL2507

Series:

GSE3188

12 Samples

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Analysis of hypoxic MCF-7 breast cancer cells depleted for hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, or both HIF alpha subunits. Results provide insight into the extent of the role of HIF-1alpha and HIF-2alpha in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL570

Series:

GSE3188

12 Samples

Download data

Analysis of MCF-7 breast cancer cells exposed to hypoxia or treated with the non-specific 2-oxoglutarate (2-OG) dependent dioxygenase inhibitor dimethyloxalylglycine. Results provide insight into the role of 2-OG dioxygenases in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 protocol sets

Platform:

GPL2507

Series:

GSE3188

6 Samples

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Analysis of MCF-7 breast cancer cells exposed to hypoxia or treated with the non-specific 2-oxoglutarate (2-OG) dependent dioxygenase inhibitor dimethyloxalylglycine. Results provide insight into the role of 2-OG dioxygenases in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 protocol sets

Platform:

GPL96

Series:

GSE3188

9 Samples

Download data

(Submitter supplied) What governs the transition between the two HIFs (the HIF switch) and the role of miRNAs in this regulation is not completely clear. Using genome-wide expression studies on the miRNA content of RNA-induced silencing complex (RISC) in HUVECs exposed to hypoxia compared to the global miRNA-Seq analysis revealed dramatic differences between the two. In our analyses, compared the miRNA and mRNA levels in RISC at 2 hours (mainly HIF-1 driven), 8 hours (HIF-1 and HIF-2 elevated), and 16 hours (mainly HIF-2 driven) in a gene ontology context. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

24 Samples

Download data: XLSX

(Submitter supplied) What governs the transition between the two HIFs (the HIF switch) and the role of miRNAs in this regulation is not completely clear. Using genome-wide expression studies on the miRNA content of RNA-induced silencing complex (RISC) in HUVECs exposed to hypoxia compared to the global miRNA-Seq analysis revealed dramatic differences between the two. In our analyses, compared the miRNA and mRNA levels in RISC at 2 hours (mainly HIF-1 driven), 8 hours (HIF-1 and HIF-2 elevated), and 16 hours (mainly HIF-2 driven) in a gene ontology context. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

14 Samples

Download data: XLSX

(Submitter supplied) K562 cells were grown at different oxygen levels (21%, 5%, 1%) and samples were collected at 6hr, 24hr, 3day, and 6day time points (triplicate per time point).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

36 Samples

Download data: TXT