GEO DataSets

(Submitter supplied) Peripheral blood transcriptome is an important intermedia data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four weeks old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood gene expression profile before treatment (d0) and at 2 and 7 day post inoculation (dpi) using deep sequencing technology.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

18 Samples

Download data

(Submitter supplied) Peripheral blood transcriptome is an important intermediate data source for investigation of the mechanism of Salmonella invasion, proliferation, and transmission but its development in pig is quite limited. We challenged four-week-old piglets (Duroc × Landrace × Yorkshire crossbred) with Salmonella enterica serovar Typhimurium LT2 and investigated the peripheral blood miRNA expression profile before treatment (d0) and at 2 days post inoculation (dpi) using deep sequencing technology.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

6 Samples

Download data: TXT

(Submitter supplied) Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of this gram-negative bacteria in such pigs is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonella shedding following experimental inoculation, we have initiated the first analysis of the whole blood transcriptional response induced by Salmonella. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Dataset:

GDS4246

Platform:

GPL3533

20 Samples

Download data: CEL

Analysis of peripheral blood from Salmonella enterica serovar Typhimurium (ST)-inoculated animals differing in Salmonella shedding phenotype at 0 and 2 days post-inoculation (dpi) . Fecal shedding of asymptomatic, Salmonella-carriers can lead to foodborne illness.

Organism:

Sus scrofa

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 10 individual, 2 time sets

Platform:

GPL3533

Series:

GSE27000

20 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by array

Platforms:

GPL15403 GPL14875

17 Samples

Download data: TSV, TXT

(Submitter supplied) This study reports on infection-inducible miRNAs in zebrafish. Using a custom-designed microarray platform for miRNA expression we found that miRNAs of the miR-21, miR-29, and miR-146 families were commonly induced by infection of zebrafish embryos with Salmonella typhimurium and by infection of adult fish with Mycobacterium marinum.

Organism:

Danio rerio

Type:

Non-coding RNA profiling by array

Platform:

GPL15403

9 Samples

Download data: TXT

(Submitter supplied) We used zebrafish embryos as an in vivo system to investigate the role of the microRNA-146 family (consisting of 2 members miR-146a and miR-146b) in the innate immune response to S. typhimurium infection. To determine the role of miR-146 microRNAs in the response to S. typhimurium infection we used Illumina RNA sequencing to compare the mRNA expression profiles of control embryos versus embryos with knockdown of miR-146a and miR-146b. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14875

8 Samples

Download data: TSV

(Submitter supplied) White leghorn layers were infected with Salmonella Enteritidis. The cecum were collected at 7 days post infection for total RNA isolation. The significantly expressed microRNAs between infected and non-infected chickens were identified through Solexa sequencing technology.

Organism:

Gallus gallus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16133

6 Samples

Download data: TXT

(Submitter supplied) High-density gene expression profile of 14,151+ unique genes for 6 normal porcine neutrophild and 6 porcine neutrophils samples infected by Salmonella using deep sequencing technology.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

12 Samples

Download data: TXT

(Submitter supplied) To understand the host transcriptional response to S. enterica serovar Choleraesuis (S. Choleraesuis), the first generation Affymetrix porcine GeneChip® was used to identify differentially expressed genes in the mesenteric lymph nodes responding to infection at acute (8 hours (h), 24h, 48h post-inoculation (pi)) and chronic stages (21 days (d) pi) The objectives of this study were to identify and examine the stereotypical gene expression response within the host mesenteric lymph nodes to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL16044 GPL16045

19 Samples

Download data: GPR, TXT

(Submitter supplied) The host response to S.typhimurium infections in ileum was studied after infection of piglets. mRNA and miRNA expression studies were performed to identify interactions of miRNAs and regulated mRNAs in context of mammalian intestinal Salmonella infection using a piglet model.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16045

9 Samples

Download data: GPR

(Submitter supplied) The host response to S.typhimurium infections in ileum was studied after infection of piglets. mRNA and miRNA expression studies were performed to identify interactions of miRNAs and regulated mRNAs in context of mammalian intestinal Salmonella infection using a piglet model.

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by array

Platform:

GPL16044

10 Samples

Download data: TXT

(Submitter supplied) Characterization of the zebrafish embryonic host response to systemic bacterial infection with Salmonella typhimurium wild type strain (SL1027) and its isogenic LPS O-antigen mutant Ra (SF1592) by means of a time-resolved global expression analysis.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL7735

36 Samples

Download data: XML

(Submitter supplied) Purpose: The goal of this study is to measure how steady state RNA and alternatively spliced transcript abundance levels are impacted by loss of specific RNA-binding proteins as part of macrophage transcriptional activation upon pathogen sensing.  Methods: SRSF 1, 2, 6, 7, 9, and hnRNP C, F, K, U were knockdown using shRNA in RAW 264.7 macrophages. Macrophages were collected as uninfected (resting) cells and infected with Salmonella Typhimuirum for 4HR. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

72 Samples

Download data: TXT

(Submitter supplied) The poly(A)+ and poly(A)− fractions of interacting and non-interacting cells were used for distinct library preparation of interacting and non-interacting prokaryotic pathogen and eukaryotic host cells by deepSuperSAGE. Sequencing was performed with the Illumina HiSeq 2000 platform, and one point of time post infection (early interaction) was additionally prepared by Massive Analysis of cDNA Ends (MACE) as alternative tag-based library preparation method.

Organism:

Salmonella enterica; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11154 GPL19227 GPL19226

12 Samples

Download data: CSV

(Submitter supplied) A first generation Affymetrix GeneChip® Porcine genome array was used to profile the gene expression in porcine mesenteric lymph nodes over a time course of infection with S. Typhimurium, including the acute (8 hours post inoculation (hpi), 24 hpi, 48 hpi) and chronic (21 days post-inoculation (dpi)) stages of infection. Our objectives were to 1) identify and examine the stereotypical gene expression response within host MLN to S. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

15 Samples

Download data: CEL, EXP

(Submitter supplied) In vivo microarray study of global gene expression changes in peripheral blood mononuclear cells (PBMCs) of German Landrace pigs during the innate immune response to porcine reproductive and respiratory syndrome virus vaccination.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platforms:

GPL21514 GPL16493

42 Samples

Download data: CEL

(Submitter supplied) Purpose: The mycotoxin zearalenone (ZEN) poses a risk to animal health because of its estrogenic effects. Diagnosis of ZEN-induced disorders in animal production remains challenging due to the lack of appropriate non-invasive biomarkers. Thus, we combined untargeted and targeted transcriptomics approaches to investigate the effects of ZEN on the microRNA expression in porcine uterus, jejunum and serum. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20983

36 Samples

Download data: XLSX