GEO DataSets

(Submitter supplied) Neisseria meningitidis is an obligate commensal colonising the human nasopharynx and occasionally invades the bloodstream causing life-threatening meningitis and septicaemia. The gene NMB0419 on the genome of N. meningitidis MC58 encodes a putative Sel1-like repeat (SLR) containing protein, which has been implicated in mediating meningococcal invasion of epithelial cells. We prepared RNA samples from N. more...

Organism:

Neisseria meningitidis MC58

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21879

6 Samples

Download data: TXT

(Submitter supplied) In this study wild-type, fur mutant, and complemented fur mutant strains of the human pathogen Neisseria gonorrhoeae F62 were grown under high (100 uM iron) or low (100 uM desferal) iron conditions to identify genes whose expression was regulated by iron and/or Fur

Organism:

Neisseria gonorrhoeae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20721

10 Samples

Download data: TXT

(Submitter supplied) Pathogenicity islands and plasmids encode genes for pathogenesis of various Escherichia coli pathotypes.Although there is a basic understanding of the contribution of these virulence factors to disease, less is known about variation in regulatory networks in determining disease phenotypes. Here we dissected aregulatory network directed by the conserved iron homeostasis regulator, Ferric Uptake Regulator (Fur), inuropathogenic E. more...

Organism:

Escherichia coli CFT073

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18955

19 Samples

Download data: TXT, WIG

(Submitter supplied) In Neisseria meningitidis iron responsive gene regulation is mediated primarily by the Ferric Uptake Regulator (Fur) protein. When complexed with iron, Fur represses gene expression by preventing transcription initiation. Fur can also indirectly activate gene expression via the repression of regulatory small RNAs (sRNA). One such Fur-and iron-regulated sRNA, NrrF, was previously identified in N. meningitidis and shown to repress expression of the sdhA and sdhC genes encoding subunits of the succinate dehydrogenase complex. more...

Organism:

Neisseria meningitidis MC58

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL10038

18 Samples

Download data: PAIR

(Submitter supplied) Whole genome DNA microarray of Neisseria meningitidis serogroup B strain MC58 and the isogenic mutant strain deficient of the transcriptional regulator FarR, MC58 ∆farR.

Organism:

Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL8787

6 Samples

Download data: GPR

(Submitter supplied) Neisseria meningitidis, a commensal β-proteobacterium of the human nasopharynx, constitutes a worldwide leading cause of sepsis and epidemic meningitis. The molecular basis for their "accidental" pathogenicity is still not fully understood. Here, we show that knock-out strains lacking the Cas9 protein are impaired in the adhesion to human nasopharyngeal cells which constitutes a central step in the pathogenesis of invasive meningococcal disease. more...

Organism:

Neisseria meningitidis 8013

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24628

8 Samples

Download data: WIG

(Submitter supplied) To get a high resolution understanding of the effect of Fur on global gene expression, we compared by high-resolution RNAseq the transcriptomes of a wild-type E. coli K-12 strain and its Fur deletion derivative grown in minimal medium with or without supplementation of iron. Three independent total RNA extraction and RNAseq assays were performed for each strain in each condition.

Organism:

Escherichia coli

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25368

12 Samples

Download data: TXT

(Submitter supplied) Differences in the transcriptional profile of the menigococcal strains MC58 and α710 were compared upon growth in PPM+ and on exposure to human saliva, whole blood and cerebrospinal fluid

Organism:

Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL8787

24 Samples

Download data: GPR

(Submitter supplied) While global transcription factors (TFs) have been studied extensively in Escherichia coli model strains, conservation and diversity in TF regulation between strains is still unknown. Here we use a combination of ChIP-exo--to define ferric uptake regulator (Fur) binding sites--and differential gene expression--to define the Fur regulon in nine E. coli strains. We then define a pan-regulon consisting of 469 target genes that includes all Fur target genes in all nine strains. more...

Organism:

Escherichia coli

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25368

4 Samples

Download data: GFF

(Submitter supplied) Regulons for many transcription factors have been elucidated in model strains leading to an understanding of their role in producing physiological states. Comparative analysis of a regulon and its target genes between different strains of the same species is lacking. Ferric uptake regulator (Fur), involved in iron homeostasis, is one of the most conserved TFs, and is present in a wide range of bacteria. more...

Organism:

Salmonella enterica; Escherichia coli O157:H7; Escherichia coli; Escherichia coli K-12

Type:

Expression profiling by high throughput sequencing

4 related Platforms

36 Samples

Download data: CSV

(Submitter supplied) Regulons for many transcription factors have been elucidated in model strains leading to an understanding of their role in producing physiological states. Comparative analysis of a regulon and its target genes between different strains of the same species is lacking. Ferric uptake regulator (Fur), involved in iron homeostasis, is one of the most conserved TFs, and is present in a wide range of bacteria. more...

Organism:

Escherichia coli; Escherichia coli K-12; Escherichia coli O157:H7; Salmonella enterica

Type:

Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms

36 Samples

Download data: GFF

(Submitter supplied) We looked at host gene expression signatures common and specfic to occasionally pathogenic N. meningitidis and commensal N. lactamica. We hypothesise that similar host cell responses during early interactions with N. meningitidis and N. lactamica could inform common mechanisms of colonisation of commensals while differential host cell responses could be capable of altering the outcome of the colonisation process.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5104

192 Samples

Download data: TXT

(Submitter supplied) The zur regulon in Neisseria meningitidis was elucidated in the strain MC58 using a zur knockout strain and conditions which activate Zur ( zinc supplementation in the medium)

Organism:

Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL8787

11 Samples

Download data: GPR, TXT

(Submitter supplied) Intracellular zinc concentration needs to be maintained within strict limits due to its toxicity at high levels, and this is achieved by a finely regulated balance between uptake and efflux. Many bacteria use the Zinc Uptake Regulator Zur to orchestrate zinc homeostasis, but little is known regarding the transport of this metal across the bacterial outer membrane. In this work we determined the C. crescentus Zur regulon by global transcriptional and in silico analyses. more...

Organism:

Caulobacter vibrioides CB15; Caulobacter vibrioides NA1000

Type:

Expression profiling by array

Platform:

GPL10469

3 Samples

Download data: TXT

(Submitter supplied) Genetically programmed deaths play important roles in the biology of unicellular prokaryotic cells. Some gene complexes force their maintenance on the host bacterial cells by killing cells that have lost them. This form of programmed death called post-segregational killing or genetic addiction is brought about by several Type II restriction-modification gene complexes, through restriction attack on the undermethylated chromosome, and underlie their behavior as selfish mobile elements. more...

Organism:

Escherichia coli; Escherichia coli K-12

Type:

Expression profiling by array

Platform:

GPL199

24 Samples

Download data: CEL

(Submitter supplied) Gluconacetobacter diazotrophicus has been the focus of several studies aiming to understand the mechanisms behind this endophytic diazotrophic bacterium. The present study is the first global analysis of the early transcriptional response of exponentially growing G. diazotrophicus to iron, an essential cofactor for many enzymes involved in various metabolic pathways. RNA-seq, targeted gene mutagenesis and computational motif discovery tools were used to define the G. more...

Organism:

Gluconacetobacter diazotrophicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29580

6 Samples

Download data: XLSX

(Submitter supplied) 4 pan-Neisseria arrays examining the gonococcal response to iron availability. Dual channel (Cy3/Cy5) arrays, using random nonamers for cDNA generation. Arrays scanned with ScanArray ExpressHT microarray scanner. Data analyzed using ArrayVision 7.0 and compiled in GeneSpring. Keywords: other

Organism:

Neisseria gonorrhoeae

Type:

Expression profiling by array

Platform:

GPL1791

4 Samples

Download data

(Submitter supplied) Background: Patients developing meningococcal septic shock reveal very high levels of Neisseira meningitidis and endotoxin in the circulation and organs, leading to acute cardiovascular, pulmonary and renal failure, coagulopathy and a high case fatality rate within 24 hours. Objective: To investigate transcriptional profiles in heart, lungs, kidneys, liver and spleen and immunostain key inflammatory cells and proteins in post mortem formalin-fixed, paraffin-embedded (FFPE) tissue samples from meningococcal septic shock patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

44 Samples

Download data: CEL, XLSX

(Submitter supplied) The overall goals and objectives of this study are to investigate the transcriptomics of Neisseria gonorrhoeae using RNA-seq. This work will look at gene expression, start points of transcription, transcriptional termination, and differences between these in different conditions and between strains and growing cultures over time.

Organism:

Neisseria gonorrhoeae NCCP11945

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20920

1 Sample

Download data: TXT

(Submitter supplied) We report for the first time movement of Correia Repeat Enclosed Elements, through inversion of the element at its chromosomal location. Analysis of Ion Torrent generated genome sequence data from Neisseria gonorrhoeae strain NCCP11945 passaged for 8 weeks in the laboratory under standard conditions and stress conditions revealed a total of 37 inversions: 24 were exclusively seen in the stressed sample; 7 in the control sample; and the remaining 3 were seen in both samples. more...

Organism:

Neisseria gonorrhoeae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18844

2 Samples

Download data: TXT