GEO DataSets

(Submitter supplied) Investigation of parasite (T. annulata) gene expression over the course of the life-cycle (sporozoite->macroschizont->merozoite->piroplasm). The study focused on the expression of known and putative transcription factors, in particular members of the ApiAP2 gene family. Up-stream motifs associated with stage-specifically expressed genes were identified during the course of the analysis.

Organism:

Theileria annulata; Bos taurus

Type:

Expression profiling by array

Platform:

GPL20733

20 Samples

Download data: TXT

(Submitter supplied) During the progress through its complex life cycle, the malaria parasite requires strict control of gene expression but the mechanism controlling this process remain poorly understood and unexplored for therapeutic purposes. A group of the apicomplexa-specific putative transcription factors with AP2 DNA binding domain (apiAP2) has been identified as the major regulators of the parasite transcriptome at multiple stages. more...

Organism:

Plasmodium berghei

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20293

62 Samples

Download data: TXT

(Submitter supplied) Plasmodium parasites are reliant on the Apicomplexan AP2 (ApiAP2) transcription factor family to regulate gene expression programs. AP2 DNA binding domains have no homologs in the human or mosquito host genomes, making them potential antimalarial drug targets. Using an in-silico screen to dock thousands of small molecules into the crystal structure of the AP2-EXP (Pf3D7_1466400) AP2 domain (PDB:3IGM), we identified compounds that interact with this domain. more...

Organism:

Plasmodium falciparum

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL21078 GPL26920

8 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) Transcriptional profiling of gametocyte non-producer lines in Plasmodium berghei Transcriptome of gametocyte non producer lines (natural and genetic KO) and parental (820) lines. The aim of the study was to identify key genes involved in the decision to commit to gametocytogenesis in Plasmodium berghei. These microarrays compare naturally selected lines that do not produce gametocytes, and the parental line and additionally a genetic knock out of AP2-G PBANKA_143750. more...

Organism:

Plasmodium berghei

Type:

Expression profiling by array

Platform:

GPL18005

28 Samples

Download data: TXT

(Submitter supplied) Commitment to and completion of sexual development are essential for malaria parasites to be transmitted through mosquitoes. The molecular mechanism(s) responsible for these processes however, remain largely unknown. We have identified two transcription factors (both belonging to the AP2 family) essential for gametocytogenesis. AP2-G mutants are characterised by a complete inability to produce gametocytes. more...

Organism:

Plasmodium berghei

Type:

Expression profiling by array

Platform:

GPL18005

8 Samples

Download data: TXT

(Submitter supplied) Overexpression of DDHA-AP2IX-9 under a tubulin promoter in type III CTG strain. Differential expression analysis under addition of shield-1 and comparison to the non-bradyzoite induced parental culture revealed a global downregulation of the bradyzoite gene expression program including canonical markers like BAG1.

Organism:

Toxoplasma gondii

Type:

Expression profiling by array

Platform:

GPL16542

11 Samples

Download data: CEL

(Submitter supplied) Transcriptomic analyses revealed the gene expression changes after AP2XII-1 depletion. To see which of these differentially expressed genes are directly targeted by AP2XII-1, we used chromatin immunoprecipitation, followed by deep sequencing (ChIP-Seq) to assess the binding positions of AP2XII-1 in the parasite’s genome.

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33297

4 Samples

Download data: BW

(Submitter supplied) To investigate transcriptional role of TgAP2XII-1 on type II Toxoplasma gondii (ME49 Tir1), an auxin inducible degron system was introduced to control the protein expression of TgAP2XII-1 and the target protein could be totally degradated in 3h after adding IAA.

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33297

6 Samples

Download data: TXT

(Submitter supplied) To investigate transcriptional role of TgAP2XII-1 on type I Toxoplasma gondii (RHΔhxgprt Tir1), an auxin inducible degron system was introduced to control the protein expression of TgAP2XII-1 and the target protein could be totally degradated in 3h after adding IAA.

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33110

9 Samples

Download data: TXT

(Submitter supplied) Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

6 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Plasmodium falciparum

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26835

192 Samples

Download data: BED, BW

(Submitter supplied) Genome-wide occupancy of sixteen PfApiAP2 transcription factors and PfHP1 throughout the intraerythrocytic cycle

Organism:

Plasmodium falciparum

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26835

144 Samples

Download data: BED, BW

(Submitter supplied) Effects of PfApiAP2 knockouts on gene expression in the intraerythrocytic cycle

Organism:

Plasmodium falciparum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26835

48 Samples

Download data: CSV

(Submitter supplied) Transcriptional profiling of transgenic P. falciparum asexual blood stage parasites of the transgenic strain 3D7/DDGFP-PfAP2-HC at five time points during intra-erythrocytic parasite development. The DD (FKBP destabilisation domain) allows for the conditional expression of fusion proteins: DD fusion proteins are rapidly degraded or stably expressed in absence or presence of the stabilising ligand Shield-1, respectively (Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ. more...

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL15130

10 Samples

Download data: GPR

(Submitter supplied) In this study we have investigated PfAP2-HC (PF3D7_1456000), a protein that was identified by co-immunoprecipitation with PfHP1 coupled with liquid chromatography-tandem mass spectrometry. PfAP2-HC belongs to the ApiAP2 family, the main transcription factor family in Apicomplexan parasites. We have confirmed that AP2-HC colocalises with HP1 with the use of immunofluorescence assays and chromatin immunoprecipitation-sequencing. more...

Organism:

Plasmodium falciparum 3D7

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25935

17 Samples

Download data: BEDGRAPH