GEO DataSets

(Submitter supplied) Acute cellular rejection occurs frequently during the first few weeks following liver transplantation. During this period its molecular phenotype is confounded by pro-inflammatory events elicited by surgery, ischemia-reperfusion injury and early post-transplant complications. To unambiguously define the molecular profile associated with rejection we collected sequential biological specimens from liver transplant patients at least 3 years after transplantation who developed rejection while enrolled in trials of intentional immunosuppression withdrawal

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8136

74 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL11154 GPL5175

153 Samples

Download data: CEL, TXT

(Submitter supplied) Histological features of acute rejection can be detected in surveillance biopsies despite stable graft function and can negatively impact graft outcomes. However, routine surveillance biopsies for detection of subclinical rejection are not generally performed due to potential risks and costs associated with repeated biopsies. Noninvasive biomarkers are required to facilitate early detection of acute rejection and borderline changes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

65 Samples

Download data: CEL

(Submitter supplied) Histological features of acute rejection can be detected in surveillance biopsies despite stable graft function and can negatively impact graft outcomes. However, routine surveillance biopsies for detection of subclinical rejection are not generally performed due to potential risks and costs associated with repeated biopsies. Noninvasive biomarkers are required to facilitate early detection of acute rejection and borderline changes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

88 Samples

Download data: TXT

(Submitter supplied) Acute rejection (AR) in renal transplantation is an established risk factor for reduced allograft survival, and still occurs in 10-20% of allograft recipients despite standard of care immunosuppression. This suggests molecular pathways exist which are inadequately suppressed by current therapy. We describe for the first time, integrative network- based computational strategies incorporating genotyping data to identify key driver genes (KDGs) amongst networks of perturbed transcripts in AR, which may serve as therapeutic targets.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

52 Samples

Download data: CEL

(Submitter supplied) Complications due to long-term administration of immunosuppressive therapy increase the morbidity and mortality of liver transplant recipients. Discontinuation of immunosuppressive drugs in recipients spontaneously developing operational tolerance could substantially lessen this burden. However, this strategy results in the development of rejection in a high proportion of recipients who require lifelong immunosuppression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

71 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL6947

134 Samples

Download data: CEL

(Submitter supplied) In clinical organ transplantation complete cessation of immunosuppressive therapy can be successfully accomplished in selected recipients providing a proof-of-principle that allograft tolerance is attainable in humans. The intra-graft molecular pathways associated with human allograft tolerance, however, have not been comprehensively studied before. In this study we analyzed sequential liver tissue samples collected from liver recipients enrolled in a prospective multicenter immunosuppressive withdrawal clinical trial. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

110 Samples

Download data: TXT

(Submitter supplied) In clinical organ transplantation complete cessation of immunosuppressive therapy can be successfully accomplished in selected recipients providing a proof-of-principle that allograft tolerance is attainable in humans. The intra-graft molecular pathways associated with human allograft tolerance, however, have not been comprehensively studied before. In this study we analyzed sequential liver tissue samples collected from liver recipients enrolled in a prospective multicenter immunosuppressive withdrawal clinical trial. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Immune responses induced by ongoing and/or past infections prevent the establishment of transplantation tolerance in experimental animal models. How host-pathogen interactions influence allograft tolerance in humans has not been investigated before. The spontaneous development of operational tolerance in liver transplant recipients with chronic hepatitis C virus (HCV) infection constitutes a unique setting to address this question. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

50 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: Assessment of gene expression in peripheral blood may provide a noninvasive screening test for allograft rejection. We hypothesized that changes in peripheral blood expression profiles would correlate with biopsy-proven rejection and would resolve after treatment of rejection episodes. METHODS AND RESULTS: We performed a case-control study nested within a cohort of 189 cardiac transplant patients who had blood samples obtained during endomyocardial biopsy (EMB). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2386

Platform:

GPL96

21 Samples

Download data: CEL

Analysis of peripheral blood from cardiac transplant patients with allograft rejection and from the same patients after resolution of rejection with augmented immunosuppression. Results provide insight into potential use of peripheral blood profiling for detection of cardiac allograft rejection.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 individual sets

Platform:

GPL96

Series:

GSE5967

21 Samples

Download data: CEL

(Submitter supplied) Recurrent hepatitis C virus (rHCV) is universal post-liver transplantation (LT), with accelerated fibrosis rates compared to non-transplanted patients. rHCV is associated with increased mortality and morbidity post-transplant and is a leading indication for re-transplantation. We hypothesized that miRNA expression profiles from liver grafts can distinguish severity of HCV recurrence and differentiate this from acute cellular rejection (ACR). more...

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL14613

29 Samples

Download data: CEL

(Submitter supplied) mmunosuppressive drugs can be completely withdrawn in up to 20% of liver transplant recipients, commonly referred to as ‘operationally’ tolerant. Immune characterization of these patients, however, has not been performed in detail, and we lack tests capable of identifying tolerant patients among recipients receiving maintenance immunosuppression. In the current study we have analyzed a variety of biological traits in peripheral blood of operationally tolerant liver recipients in an attempt to define a multiparameter ‘fingerprint’ of tolerance. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3282

Platform:

GPL570

17 Samples

Download data: CEL

Analysis of peripheral blood mononuclear cells from operationally tolerant liver transplant patients. Operationally tolerant patients are fully immunocompetent but accept grafts without chronic immunosuppression. Results identify a molecular signature for operational tolerance.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 specimen sets

Platform:

GPL570

Series:

GSE11881

17 Samples

Download data: CEL

(Submitter supplied) Liver biopsy samples were obtained as part of the LITMUS trial (ClinicalTrials.gov NCT02541916). All samples were obtained from liver transplant recipients prior to immunosuppression withdrawal. Percutaneous liver biopsies were performed under local anaesthesia using an 18-gauge Jamshidi needle. A 0.5cm portion of the biopsy to be used for gene expression was stored in RNAlater (Qiagen) for 24 hours at 4 °C and then transferred to -80 °C. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

17 Samples

Download data: TSV

(Submitter supplied) In the present work, we have used whole genome expression profiling of peripheral blood samples from 51 patients with biopsy-proven acute kidney transplant rejection and 24 patients with excellent function and biopsy-proven normal transplant histology. The results demonstrate that there are 1738 probesets on the Affymetrix HG-U133 Plus 2.0 GeneChip representing 1472 unique genes which are differentially expressed in the peripheral blood during an acute kidney transplant rejection. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

75 Samples

Download data: CEL