GEO DataSets

(Submitter supplied) Wolfram syndrome, an autosomal recessive disorder characterized by juvenile-onset diabetes mellitus and optic atrophy, is caused by mutations in the WFS1 gene. WFS1 encodes an endoplasmic reticulum resident transmembrane protein. The Wfs1-null mice exhibit progressive insulin deficiency and diabetes. The aim of the present study was to describe the insulin secretion and transcriptome of pancreatic islets in WFS1-deficient mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15907

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18061 GPL14602

20 Samples

Download data

(Submitter supplied) Wolfram syndrome is caused by mutations in the WFS1 gene. WFS1 protein dysfunction results in a range of neuroendocrine syndromes and is mostly characterized by juvenile-onset diabetes mellitus and optic atrophy. WFS1 has been shown to participate in membrane trafficking, protein processing and Ca2+ homeostasis in the endoplasmic reticulum. In the present study we aimed to find the transcriptomic changes influenced by Wfs1 in the hypothalamus and hippocampus using RNA-sequencing. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18061

12 Samples

Download data: TXT

(Submitter supplied) Wolfram syndrome is caused by mutations in the WFS1 gene. WFS1 protein dysfunction results in a range of neuroendocrine syndromes and is mostly characterized by juvenile-onset diabetes mellitus and optic atrophy. WFS1 has been shown to participate in membrane trafficking, protein processing and Ca2+ homeostasis in the endoplasmic reticulum. In the present study we aimed to find the transcriptomic changes influenced by Wfs1 in the hypothalamus and hippocampus using RNA-sequencing. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14602

8 Samples

Download data: TXT

(Submitter supplied) Pancreatic β-cell failure induced by WFS1 deficiency is manifested in wolfram syndrome (WS). The lack of a suitable human model in WS has hampered the progress in developing new treatments. Here, human pluripotent stem cell derived pancreatic β cells (SC-β cells) harboring WFS1-deficiency and mouse model of β cell-specific Wfs1 knockout were applied to model β-cell failure in WS. Single-cell RNA sequencing of WFS1-deficient SC-β cells revealed two cell fates along pseudotime trajectory including maturation and stress branch. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30209

2 Samples

Download data: MTX, TSV

(Submitter supplied) We used microarrays to compare the global programme of gene expression in primary cultures of neurons and astrocytes. These data sets were compared to the expression profiles of other tissues, including pancreatic islets, in order to identify a specific neuroendocrine program in pancreatic islets.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) β-cell identity is determined by tightly regulated transcriptional networks that are modulated by extracellular cues, thereby ensuring β-cell adaptation to the organism’s insulin demands. We have observed in pancreatic islets that stimulatory glucose concentrations induced a gene profile that was similar to that of freshly isolated islets, indicating that glucose-elicited cues are involved in maintaining β-cell identity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4939 GDS4942

Platform:

GPL1261

26 Samples

Download data: CEL

Analysis of islets isolated from young animals and cultured at 3mM glucose (G3) for 10 hr (T0) and then challenged with 11mM glucose for 1 hr (T1) and 4 hr (T4). Islets cultured at G3 for 2 days were also examined. Results provide insight into the kinetics of molecular response of islets to glucose.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 dose, 4 time sets

Platform:

GPL1261

Series:

GSE42591

8 Samples

Download data: CEL

Analysis of islets isolated from young and aged animals and cultured for 2 days at 3 to 16mM glucose (G3 to G16). Freshly-isolated islets were also examined. Ageing is associated with functional changes in beta cells. Results provide insight into the molecular response of aged islets to glucose.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 5 dose, 2 protocol sets

Platform:

GPL1261

Series:

GSE42591

20 Samples

Download data: CEL