GEO DataSets

(Submitter supplied) Large-scale identification of secreted and membrane-associated gene products using DNA microarrays. This study is described more fully in Diehn M et al.(2000) Nat Genet 25:58-62 Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL179

1 Sample

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(Submitter supplied) Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. mRNA species bound to membrane-associated polysomes were separated from other mRNAs by sedimentation equilibrium or sedimentation velocity. The distribution of individual transcripts in the 'membrane-bound' and 'cytosolic' fractions was quantitated for thousands of genes by hybridization to DNA microarrays. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2773

1 Sample

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(Submitter supplied) RNA from MCF-7 cells was fractionated by sucrose density gradient centrifugation to separate RNA associated with membrane-bound polysomes from RNA associated with free polysomes. These two populations were hybridized in triplicate to U133A microarrays. Keywords: repeat sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS992

Platform:

GPL96

6 Samples

Download data: CEL

Identification of genes whose mRNA are translated from the endoplasmic reticulum (ER) in the breast cancer cell line MCF-7. Secreted and membrane-bound proteins are preferentially translated in the ER. Results provide insight into the association of membrane-bound proteins and breast cancer.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL96

Series:

GSE1400

6 Samples

Download data: CEL

(Submitter supplied) Membrane-associated, integral membrane and secreted proteins are of key importance in many cellular processes. For most of the 28 952 predicted proteins in Arabidopsis, the actual subcellular localisation has not been demonstrated experimentally. So far, their potential membrane-association has been deduced from algorithms that predict transmembrane domains and signal peptides. However, the comprehensiveness and accuracy of these algorithms is still limited. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL3357

12 Samples

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(Submitter supplied) Construction and Preparation of Synechocystis Spotted Microarrays: 3168 genes or putative ORFs of Synechocystis sp. PCC6803 were PCR amplified in two stages. Primary amplification was achieved using gene-specific up-stream and down-stream primers modified with adaptamer sequences and genomic DNA template. Secondary amplification was achieved using adaptamer primers and individual primary PCR product templates. more...

Organism:

Synechocystis sp. PCC 6803

1 Series

16 Samples

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(Submitter supplied) The subcellular localization of proteins is critical to their biological roles. Moreover, whether a protein is membrane-bound, secreted, or intracellular affects the usefulness of, and the strategies for, using a protein as a diagnostic marker or a target for therapy. We employed a rapid and efficient experimental approach to classify thousands of human gene products as either membrane-associated/secreted (MS) or cytosolic/nuclear (CN). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

19 Samples

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