GEO DataSets

Analysis of genital fibroblasts from 46,XY females with complete androgen insensitivity syndrome (AIS). Response of cells to dihydrotestosterone examined. Most AIS affected individuals have mutated androgen receptors (AR). Results provide insight into the role of AR in genital differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 disease state, 3 dose, 2 protocol sets

Platform:

GPL3039

Series:

GSE3871

21 Samples

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(Submitter supplied) Abstract: BACKGROUND: Androgen insensitivity syndrome (AIS) comprises a range of phenotypes from male infertility to complete feminization. Most individuals with AIS carry germline mutations of the androgen receptor (AR) that interfere with or ablate its function. As genital fibroblasts retain expression of the AR in vitro, we used genital skin fibroblasts from normal males and 46,XY females with complete AIS due to known AR mutations to gain insights into the role of the AR in human genital differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

46 Samples

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(Submitter supplied) To gain insights into the role of androgen in genital morphogenesis, we compared basal transcriptional patterns in genital fibroblasts from 46,XY individuals with either wild-type AR or germline inactivation of the AR as a result of mutation. A disease state experiment design type is where the state of some disease such as infection, pathology, syndrome, etc is studied. Keywords: disease_state_design

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

25 Samples

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(Submitter supplied) e tested the responses of normal and AIS genital fibroblasts to dihydrotestosterone (DHT), under culture conditions similar to those that were reported to produce aromatase induction in these cells [8]. Cells were treated with DHT (100-1,000 nM) both at confluency (G0) and during exponential growth, and transcript levels were assessed using DNA microarrays. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1836

Platform:

GPL3039

21 Samples

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(Submitter supplied) This gene set contains skin fibroblasts from either labia majora of 46,XY sex reversed females having complete androgen insensitivity syndrome due to inactivation mutations of the androgen receptor gene and from the scrotum of normal males. Both, labia majora and scrotum origin from the same embryological anlagen, the labioscrotal swellings. The phenotypic difference is due to androgen dependent virilization in males. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3526 GPL4761 GPL3335

15 Samples

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(Submitter supplied) This dataset contains fibroblasts from different genital and extragenital regions, mostly from 46,XY individuals. Due to proven androgen receptor gene mutations, several individuals have varying degrees of undervirilization of the external genitalia ranging from complete androgen insensitivity syndrome (CAIS) to partial androgen insensitivity syndrome (PAIS). CAIS is a completely female person despite a 46,XY karyotype. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

10 related Platforms

71 Samples

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(Submitter supplied) Endogenous and exogenous androgens induce masculinization of external genitalia through binding to the androgen receptor (AR). Although several efforts were made to determine the androgen target genes in external genitalia by analyzing transcript profiles in cultured genital skin fibroblasts, no genes except for APOD were shown to be significantly regulated by androgens. In the present study, we performed microarray analysis for genital skin fibroblasts obtained from four boys with buried penis (the control individuals) and a patient with androgen insensitivity syndrome (AIS) due to a hypomorphic mutation in AR (the AIS patient). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

12 Samples

Download data: TXT

(Submitter supplied) Human scrotum and labia majora samples treated with DHT and/or AZA A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: dihydrotestosterone (DHT), 5-aza-deoxy-cytidine (AZA), none or both Cell Line: normal scrotum or labia majora derived from complete androgen insensitivity syndrome due to inactivating mutation of the androgen receptor gene

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6557

16 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

34 Samples

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(Submitter supplied) The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL2947 GPL3039 GPL2938

24 Samples

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(Submitter supplied) LNCaP cells are an established androgen receptor expressing prostate carcinoma cell line. Human foreskin fibroblasts also expressing the androgen receptor were obtained from phenotypic normal male individuals. Cells were cultured either at confluency leading to G0 cell cycle state or while they were proliferating. Cells were either untreated, or treated with dihydrotestosterone (DHT) or ethanol (ETOH) which also served as the solvent for the DHT. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

26 Samples

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