Deep sequencing and fitness calculation for plasmids carrying the CREATE cassette from the enriched tolerant strains under different pressures by using Illumina protocol - - GEO DataSets

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Deep sequencing and fitness calculation for plasmids carrying the CREATE cassette from the enriched tolerant strains under different pressures by using Illumina protocol

(Submitter supplied) We report using global regulator libraries based on the CRISPR-enabled trackable genome engineering (CREATE) method to engineer tolerance against multiple inhibitors in Escherichia coli. Deep mutagenesis libraries were rationally designed, constructed, and screened to target 34,340 mutations across 23 global regulators.These libraries were divided into G1, G2, G3, G4 and G5 sub-groups according to different gene functions (such as active sites, DNA binding sites, and predicted functional sites) . more...

Organism:: Escherichia coli str. K-12 substr. MG1655

Type:: Other

Platform:: GPL21117
5 Samples

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Accession:: GSE200448

ID:: 200200448

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Deep sequencing and fitness calculation for plasmids carrying the CREATE cassett...
Deep sequencing and fitness calculation for plasmids carrying the CREATE cassette from the enriched tolerant strains under different pressures by using Illumina protocol
Accession: GSE200448

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