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Items: 1 to 20 of 184

1.

Identification of constitutively-expressed immune effectors in the house fly (Musca domestica) and the transcription factors that regulate them

(Submitter supplied) In this study we fed groups of female house flies one of two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) or control broth and used RNA-seq to determine differential gene expression at 4 hours post-feeding. Flies that ingested P. aeruginosa induced expression of genes encoding digestive proteins in the gut but did not induce AMPs. Flies in all treatment groups constitutively expressed some paralogs of lysozyme and AMP genes at the transcript level.
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30537
28 Samples
Download data: CSV
Series
Accession:
GSE182611
ID:
200182611
2.

Function analysis and characterisation of a novel chitinase, MdCht9, in Musca domestica

(Submitter supplied) To investigate how MdCht9 contribute to the development and digestion of Musca domestica, we silenced the chitinase gene 9 (MdCht9) by its dsRNA, while silenced the GFP as the control. Then we compared the differental expression genes between the test group and the control group.
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30537
6 Samples
Download data: XLS
Series
Accession:
GSE189491
ID:
200189491
3.

Effects of proto-Y chromososomes and temperature on house fly gene expression

(Submitter supplied) Purpose: House fly has a stable polygenic sex determination system. The male determining factor (Mdmd) is commonly found on the Y chromosome (Y^M) or the third chromosome (III^M). These proto-Y chromosomes are clinally distributed, with Y^M found most commonly in northern latitudes and III^M most commonly found at southern latitudes, hinting at possible genotype-by-temperature interactions that maintain the polymorphism. more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22576
24 Samples
Download data: CSV
Series
Accession:
GSE136188
ID:
200136188
4.

RNA-seq following knockdown of a sex determing gene in house fly

(Submitter supplied) We injected double-stranded RNA (dsRNA) into house fly embryos to knock down transformer (Md-tra), creating sex-reversed males that do not carry a Y chromosome. We also injected dsRNA trageting GFP as a sham treatment. We used RNA-seq to compare gene expression in the sex-reversed males with genotypic (normal) females (injected with dsRNA targeting GFP) and two types of genotypic (normal) males (injected with dsRNA targeting Md-tra or GFP). more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22576
24 Samples
Download data: TSV
Series
Accession:
GSE126689
ID:
200126689
5.

Effects of naturally ocurring proto-Y chromosomes on house fly gene expression

(Submitter supplied) We performed RNA-seq experiments to examine the effects of house fly proto-Y chromosomes on gene expression. Two different Y^M chromosomes were investigated, and two different III^M chromosomes were investigated. One of the Y^M genotypes also was also heterozygous for third chromosomes that do not carry a male-determining locus to evaluate the effect of third chromosomes on gene expression independent of being a proto-Y.
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22576
29 Samples
Download data: TSV
Series
Accession:
GSE126685
ID:
200126685
6.

Impacts of the Hytrosavirus on the Transcriptome of the Housefly, Musca domestica

(Submitter supplied) Hytrosaviridae family members replicate in the salivary glands (SGs) of their adult dipteran hosts and are transmitted to uninfected hosts via saliva during feeding. Despite inducing similar gross symptoms (SG hypertrophy; SGH), hytrosaviruses (SGHVs) have distinct pathobiologies, including sex-ratio distortions in tsetse flies and refusal of infected housefly females to copulate. Via unknown mechanism(s), SGHV replication in other tissues results in reduced fecundity in tsetse flies and total shutdown of vitellogenesis and sterility in housefly females. more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22576
6 Samples
Download data: TXT
Series
Accession:
GSE88939
ID:
200088939
7.

Early developmental transcriptome of Musca domestica.

(Submitter supplied) Using mRNA next generation sequencing, we analyze the transcriptome of Musca domestica embryos through five stages of early development: from syncytial blastoderm to dorsal closure stage.
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL15805
10 Samples
Download data: TXT
Series
Accession:
GSE85160
ID:
200085160
8.

Transcriptome analysis of insecticide resistant housefly strain: insights about SNPs and regulatory elements in differentially expressed cytochrome P450 genes

(Submitter supplied) The 791spin is the spinosad-selected strain derived from 791a, a laboratory strain derived from a multi-resistant field-collected sample of houseflies. The 791a strain proved highly resistant to pyrethroids and some anticholinesterases and showed some resistance to the chitin synthesis-disrupting larvicides.In order to understand the evolution of insecticide resistance, de novo assembly of a spinosad resistant housefly strain 791spin using 454 technology was initiated
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19777
8 Samples
Download data: FASTA, TXT
Series
Accession:
GSE65891
ID:
200065891
9.

Transcriptome Differences between Alternative Sex Determining Genotypes in the House Fly, Musca domestica

(Submitter supplied) Sex determination evolves rapidly, often because of turnover of the genes at the top of the pathway. The house fly, Musca domestica, has a multifactorial sex determination system, allowing us to identify the selective forces responsible for the evolutionary turnover of sex determination in action. There is a male determining factor, M, on the Y chromosome (Y^M), which is probably the ancestral state. more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19912
16 Samples
Download data: TSV
Series
Accession:
GSE67065
ID:
200067065
10.

A Whole Transcriptional Linkage Analysis of Gene Co-Regulation in Insecticide Resistant House Flies, Musca domestica

(Submitter supplied) Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. more...
Organism:
Musca domestica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL15805
4 Samples
Download data: TXT
Series
Accession:
GSE39327
ID:
200039327
11.

Female adult to male adult comparison and 1st instar larvae to adult comparisons

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Haematobia irritans
Type:
Expression profiling by array
Platform:
GPL6818
10 Samples
Download data: GPR
Series
Accession:
GSE11828
ID:
200011828
12.

Hornfly 1st instar larvae vs pooled adult + egg

(Submitter supplied) Background: The horn fly, Haematobia irritans (L.), is an obligate blood-feeding parasite of cattle and control of this pest is a continuing problem in the United States and other parts of the world. Worldwide annual economic losses attributable to this pest surpass $1 billion. The fly is becoming resistant to pesticides and new control technologies are needed by cattle producers. Results: Dominant conditional lethal gene systems are being investigated as population control technologies against agricultural insect pests. more...
Organism:
Haematobia irritans
Type:
Expression profiling by array
Platform:
GPL6818
5 Samples
Download data: GPR
Series
Accession:
GSE11364
ID:
200011364
13.

Female adult hornfly gene expression vs male adult hornfly gene expression

(Submitter supplied) Background: The horn fly, Haematobia irritans (L.), is an obligate blood-feeding parasite of cattle and control of this pest is a continuing problem in the United States and other parts of the world. Worldwide annual economic losses attributable to this pest surpass $1 billion. The fly is becoming resistant to pesticides and new control technologies are needed by cattle producers. Results: Dominant conditional lethal gene systems are being investigated as population control technologies against agricultural insect pests. more...
Organism:
Haematobia irritans
Type:
Expression profiling by array
Platform:
GPL6818
5 Samples
Download data: GPR
Series
Accession:
GSE11363
ID:
200011363
14.

Illumina NovaSeq 6000 (Musca domestica)

Organism:
Musca domestica
2 Series
34 Samples
Download data
Platform
Accession:
GPL30537
ID:
100030537
15.

Illumina NextSeq 500 (Musca domestica)

Organism:
Musca domestica
4 Series
83 Samples
Download data
Platform
Accession:
GPL22576
ID:
100022576
16.

Illumina HiSeq 2500 (Musca domestica)

Organism:
Musca domestica
1 Series
16 Samples
Download data
Platform
Accession:
GPL19912
ID:
100019912
17.

454 GS FLX Titanium (Musca domestica)

Organism:
Musca domestica
1 Series
8 Samples
Download data
Platform
Accession:
GPL19777
ID:
100019777
18.

Illumina HiSeq 2000 (Musca domestica)

Organism:
Musca domestica
2 Series
14 Samples
Download data
Platform
Accession:
GPL15805
ID:
100015805
19.

Hornfly Haematobia irritans EST array

(Submitter supplied) Hornfly Haematobia irritans EST array Protocol: Agilent sureprint technology
Organism:
Haematobia irritans
3 Series
10 Samples
Download data: TXT
Platform
Accession:
GPL6818
ID:
100006818
20.

Gut of flies fed E.coli (rep C)

Organism:
Musca domestica
Source name:
Gut of infected house flies
Platform:
GPL30537
Series:
GSE182611
Download data
Sample
Accession:
GSM5532386
ID:
305532386
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