GEO DataSets

(Submitter supplied) Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. more...

Organism:

Wolbachia endosymbiont of Brugia malayi; Brugia malayi

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28005

21 Samples

Download data: GTF, TSV

(Submitter supplied) Lymphatic filarial nematodes maintain a mutualistic association with the intracellular bacterium Wolbachia. Wolbachia populations expand following infection of the mammalian host, to support larval growth and development. Utilizing transcriptomic data from Brugia malayi over the first two weeks post-infection, we present an analysis of the biochemical pathways that are involved in Wolbachia population growth and regulation in support of larval development. more...

Organism:

Brugia malayi; Wolbachia endosymbiont strain TRS of Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26792

12 Samples

Download data: TXT

(Submitter supplied) We cultured D. immitis L3 and L4 of two laboratorial strains with different susceptibility status to macrocyclic lactone drugs in vitro. Excretory/secretory microRNAs were sequenced and analyzed.

Organism:

Dirofilaria immitis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL30171

12 Samples

Download data: XLSX

(Submitter supplied) We report our microarray analysis of Brugia malayi microfilariae-derived miRNA comparing parasite-derived EVs and supernatants Microarray analysis was performed using isolated RNA from three biological replicates of Brugia malayi microfilariae with a focus on the parasite-derived EVs and supernatant

Organism:

synthetic construct; Brugia malayi

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

9 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Staphylococcus aureus; Onchocerca volvulus; Yellow fever virus; Streptococcus sp. 'group B'; Mycobacterium tuberculosis; Homo sapiens; Dengue virus; Human immunodeficiency virus; Respiratory syncytial virus; Zika virus; Chikungunya virus; Bordetella pertussis; Plasmodium falciparum

Type:

Protein profiling by protein array

Platform:

GPL29530

804 Samples

Download data

(Submitter supplied) Breastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however, provides only limited information regarding their potential relationships with disease risk in the recipient infant. more...

Organism:

Staphylococcus aureus; Yellow fever virus; Chikungunya virus; Streptococcus sp. 'group B'; Onchocerca volvulus; Dengue virus; Human immunodeficiency virus; Respiratory syncytial virus; Zika virus; Bordetella pertussis; Mycobacterium tuberculosis; Plasmodium falciparum; Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL29530

402 Samples

Download data: TXT

(Submitter supplied) Breastfeeding provides defense against infectious disease during early life. The mechanisms underlying this protection are complex but likely include the vast array of immune cells and components, such as immunoglobulins, in milk. Simply characterizing the concentrations of these bioactives, however, provides only limited information regarding their potential relationships with disease risk in the recipient infant. more...

Organism:

Staphylococcus aureus; Streptococcus sp. 'group B'; Onchocerca volvulus; Dengue virus; Human immunodeficiency virus; Zika virus; Yellow fever virus; Chikungunya virus; Bordetella pertussis; Mycobacterium tuberculosis; Plasmodium falciparum; Homo sapiens; Respiratory syncytial virus

Type:

Protein profiling by protein array

Platform:

GPL29530

402 Samples

Download data: TXT

(Submitter supplied) The helminth Acanthocheilonema viteae serves as model organism for research on parasitic filarial nematodes. Total RNA secreted or excreted by 1500 adult female and male A. viteae over 3 weeks was isolated from culture media previously processed by differential ultracentrifugation, and subjected to miRNA sequencing.

Organism:

Acanthocheilonema viteae

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28011

2 Samples

Download data: XLSX

(Submitter supplied) Mosquito-borne helminth infections are responsible for a significant worldwide disease burden in both humans and animals. Accordingly, development of novel strategies to reduce disease transmission by targeting these pathogens in the vector are of paramount importance. We found that a strain of Aedes aegypti that is refractory to infection by Dirofilaria immitis, the agent of canine heartworm disease, mounts a stronger immune response during infection than does a susceptible strain. more...

Organism:

Aedes aegypti; Dirofilaria immitis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL27922 GPL22030

23 Samples

Download data: TXT

(Submitter supplied) The zoonotic mosquito-borne filarial nematode Dirofilaria repens causes subcutaneous and ocular infections in dogs, cats and humans. Microfilariae (mf) are taken up by mosquitoes from infected vertebrate hosts which develop in the mosquito to the infectious third stage larvae (L3). These are transmitted to new vertebrate hosts and develop over two further moults to adult worms. The aims of the project were 1) the de novo sequencing and annotation of the D. more...

Organism:

Dirofilaria repens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26325

6 Samples

Download data: FASTA, GTF, TXT

(Submitter supplied) Brugia malayi, Wolbachia (wBm), and Aedes aegypti transcriptome reads from across the B. malayi FR3 life cycle

Organism:

Aedes aegypti; Wolbachia endosymbiont of Brugia malayi; Brugia malayi

Type:

Expression profiling by high throughput sequencing

5 related Platforms

81 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: The release of small non-coding RNAs (sRNAs) has been reported in parasitic nematodes, trematodes and cestodes of medical and veterinary importance. However, little is known regarding the diversity and composition of sRNAs released by different lifecycle stages and the portion of sRNAs that persist in host tissues during filarial infection. This information is relevant to understanding potential roles of sRNAs in parasite-to-host communication, as well as to inform on the location within the host and time point at which they can be detected. more...

Organism:

Meriones unguiculatus; Litomosoides sigmodontis

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL27762 GPL27761

78 Samples

Download data: CSV

(Submitter supplied) To understand the genes regulating the molting of L3 to L4 larvae upon induction with ascorbic acid over a 10-day period in a controlled in-vitro molting model of the filarial parasite B.malayi.

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL8207

8 Samples

Download data: TXT

(Submitter supplied) Lymphatic filariasis (LF) threatens nearly 20% of the world’s population and has handicapped one-third of the 120 million people currently infected. Current control and eventual elimination of LF rely on mass drug administration (MDA) programs with three drugs: ivermectin (IVM), albendazole (ALB), and diethylcarbamazine (DEC). Only the mechanism of action of albendazole is well-understood. . To gain a better insight into antifilarial drug action, we treated gerbils with patent B. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22883

68 Samples

Download data: TXT

(Submitter supplied) We report analyses of the types, amounts and stage-dependence of microRNAsfound in D. immitis culture media recovered after incubating 800,000 microfilariae for 6 days, 500 third-stage larvae (L3) and 500 fourth-stage larvae (L4) for 7 days, as well as 40 adult females and 40 adult males for 48 hours, all separately.

Organism:

Dirofilaria immitis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15208

5 Samples

Download data: XLS

(Submitter supplied) We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22883

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

34 Samples

Download data

(Submitter supplied) In this study, we analyzed transcriptomic profiles from ivermectin-treated and -untreated B. malayi adult females using Next Generation RNA Sequencing technology at different concentrations (100 nM, 300 nM and 1 µM) and time points (24, 48, 72 h, and 5 days). Our analysis revealed altered expression of multiple genes involved in meiosis, as well as oxidative phosphorylation which were significantly down-regulated as early as 24 hours post-exposure. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

20 Samples

Download data: TXT

(Submitter supplied) In this study, we analyzed transcriptomic profiles from ivermectin-treated and -untreated B. malayi adult females using Next Generation RNA Sequencing technology at different concentrations (100 nM, 300 nM and 1 µM) and time points (24, 48, 72 h, and 5 days). Our analysis revealed altered expression of multiple genes involved in meiosis, as well as oxidative phosphorylation which were significantly down-regulated as early as 24 hours post-exposure. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

14 Samples

Download data: TXT

(Submitter supplied) We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont.

Organism:

Dirofilaria immitis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20032

12 Samples

Download data: TXT