GEO DataSets

(Submitter supplied) Cleavage and polyadenylation are the final steps of eukaryotic mRNA 3' end formation. The most critical element in humans and other model organisms is the poly(A) signal, an AAUAAA hexamer. We recently discovered that the deeply branching eukaryote – Giardia lamblia uses a different but well-defined poly(A) signal, AGURAA. To better characterize when this evolutionary shift in the poly(A) signal occurred, we performed direct RNA sequencing of four protists within the Metamonada supergroup and two outgroup protists. more...

Organism:

Giardia muris; Entamoeba histolytica; Naegleria gruberi; Giardia intestinalis; Trichomonas vaginalis; Tritrichomonas foetus

Type:

Expression profiling by high throughput sequencing

6 related Platforms

20 Samples

Download data: TSV

(Submitter supplied) To investigate the transcriptional responses of intestinal epithelial cells and Giardia intestinalis, assemblage A isolate WB-C6, trophozoites during infection, we infected human enteroids with preconditioned trophozoites for 1h and 3h. Giardia intestinalis trophozoites were preconditioned before the infection with either DMEM/F-12 or DMEM/F-12 supplemented with 10% FBS to modify the trophozoites’ fitness.

Organism:

Giardia intestinalis assemblage A; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL32949 GPL32948

24 Samples

Download data: TXT

(Submitter supplied) 3'-end sequencing and Oxford Nanopore Technologies long-read sequencing of trophozoite RNA to define mRNA cleavage sites

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29832 GPL29833

4 Samples

Download data: BED, TSV

(Submitter supplied) RNA-seq data from Giardia intestinalis WB trohozoites 7h into encysting Giardia cells infecting human Caco-2 cells

Organism:

Giardia intestinalis; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL25228 GPL28025 GPL16791

13 Samples

Download data: FA, TXT

(Submitter supplied) In this study we compare the baseline transcriptomic changes two resistant lines show without drug in the media compared to both the line they are derived from as well as a revertant.

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27706

20 Samples

Download data: FA, TXT

(Submitter supplied) In this study we analyzed how gene expression is regulated through the process of encystation in Giardia

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27706

35 Samples

Download data: FA, TXT

(Submitter supplied) Transcriptional profiling of Giardia lamblia WB comparing control 5pac_5n cells with 15279 cells. (5pac_5n also know as 5'∆5N-Pac) (15279 also know as pPSpo11)

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30814

2 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPMBF1 cells. Multiprotein bridging factor 1 (MBF1) gene family proteins are involved in cell growth and differentiation in various organisms. We asked whether Giardia MBF1-like protein can induce various endogenous genes. We used epitope-tagged system to overexpress Giardia MBF1 by constructing and transfecting pPMBF1, a plasmid for expressing MBF1, to trophozoites. more...

Organism:

Giardia intestinalis

Type:

Expression profiling by array

Platform:

GPL13962

1 Sample

Download data: TXT

(Submitter supplied) RNASequencing and RNA level measurements of G. lamblia transcripts during trophozite stage to compare expression between genes of interest

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25228

2 Samples

Download data: TSV

(Submitter supplied) Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPTop3b cells.

Organism:

Giardia intestinalis

Type:

Expression profiling by array

Platform:

GPL13962

1 Sample

Download data: TXT

(Submitter supplied) RNA-seq data from Giardia intestinalis WB trohozoites infecting human Caco-2 cells

Organism:

Giardia intestinalis; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28025 GPL16791 GPL25228

15 Samples

Download data: FA, TXT

(Submitter supplied) This study use rna-seq method to examin the effect of iron depletion.

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25228

9 Samples

Download data: FA, TXT

(Submitter supplied) Giardia lamblia is one of most common agents causing persistent abdominal symptoms in developed and developing countries. There are several diagnostic methods for Giardia infection, but none are optimal. In this study our aim was to find a new method based on Giardia microRNA (miRNA) that would contribute to the currently available diagnostic methods of giardiasis. Profiling Giardia small RNAs by deep sequencing revealed that the previously reported putative miR5 and miR6 are expressed in several G. more...

Organism:

Giardia intestinalis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25228

10 Samples

Download data: XLSX

(Submitter supplied) To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the two stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

Organism:

Giardia lamblia ATCC 50803

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26206

6 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPTopoII cells.

Organism:

Giardia intestinalis

Type:

Expression profiling by array

Platform:

GPL13962

1 Sample

Download data: TXT

(Submitter supplied) We have performed strand-specific RNA-seq of trophozoites from four different Giardia intestinalis strains (A=WB and AS175, B=GS, E=P15).

Organism:

Giardia intestinalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15348

5 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPPax2 cells.

Organism:

Giardia intestinalis

Type:

Expression profiling by array

Platform:

GPL13962

1 Sample

Download data: TXT, XLS

(Submitter supplied) To investigate the magnitude of the transcriptional changes occurring during the life cycle of Giardia lamblia we compared the transcriptome of trophozoites and cysts. Cysts were found to possess a much smaller transcriptome, both in terms of mRNA diversity and abundance. Genes encoding proteins related to ribosomal functions are highly over-represented. In cysts which have lost infectivity the transcriptome is further depleted. more...

Organism:

Giardia intestinalis

Type:

Expression profiling by array

Platform:

GPL11228

9 Samples

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(Submitter supplied) Giardia gene expression is being examined using Serial Analysis of Gene Expression (SAGE) to monitor genome-wide levels of messenger RNA (mRNA) expression throughout Giardia's life cycle. Examination of genome-wide gene expression patterns will provide a coherent picture of activation and inactivation of biological pathways. This research will provide a comprehensive understanding of changes in giardial gene expression in response to important host physiological signals and will serve as a valuable model for study of other parasites and complex eukaryotes, such as yeast and animals. more...

Organism:

Giardia intestinalis

Type:

Expression profiling by SAGE

Platform:

GPL5454

10 Samples

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