GEO DataSets

(Submitter supplied) Comparative hybridization analysis Microarray-based genomic hybridization was used here as a high-throughput analog to traditional southern hybridization, which is the classical standard method for detecting specific DNA fragments in a genome.

Organism:

Vigna angularis; Vigna unguiculata

Type:

Genome variation profiling by array

Platform:

GPL24932

1 Sample

Download data: TXT

(Submitter supplied) Microarray hybridizations of a long-pod and a short-pod pool, each consisting of RNA from seven independent genotypes, were performed to reveal the gene regulatory basis underlying the genotypic pod length difference.

Organism:

Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL21758

12 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in two asparagus bean accesions B47 and B128 under drought stress, compared to well-watered conditions. Two organs, leaf and root, were sampled for each accesion under both well-watered and drought conditions.

Organism:

Vigna unguiculata subsp. sesquipedalis; Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL19461

24 Samples

Download data: PAIR

(Submitter supplied) Background. Cowpea, Vigna unguiculata L. Walp., is one of the most important food and forage legumes in the semi-arid tropics. While most cowpea accessions are susceptible to the root parasitic weed Striga gesnerioides, several cultivars have been identified that show race-specific resistance. Cowpea cultivar B301 contains the RSG3-301 gene for resistance to S. gesnerioides race SG3, but is susceptible to race SG4z. more...

Organism:

Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL15808

12 Samples

Download data: XYS

(Submitter supplied) To identify novel microRNAs that are associated with drought tolerance in two different cowpea genotypes, we generated small RNA sequences from adult cowpea plants under control and dought stress treatments. Over 79 million raw reads were generated and numerous novel microRNAs are identified, including some associated with drought tolerance.

Organism:

Vigna unguiculata

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11377

4 Samples

Download data: TXT

(Submitter supplied) Screening cDNA clones from SSH library by hybridization with cDNA used to construct the library. Treated samples were drought-stressed cowpea plants, and control samples were cowpea plants subjected to a standard watering regime. cDNA clones from forward and reverse libraries are spotted on the same array, but data from each library were analysed separately after normalization using SSHscreen software (http://microarray.up.ac.za/SSHscreen ) to calculate Enrichment Ratio 3 (ER3) and Enrichment Ratio 2 (ER2) values for each clone. more...

Organism:

Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL10024

12 Samples

Download data: GPR, TXT

(Submitter supplied) Certain alpha- and beta-proteobacteria, the rhizobia, are able to infect legume roots, elicit root nodules, and live therein as endosymbiotic, nitrogen-fixing bacteroids. Host recognition and specificity are the results of consecutive programming events in bacteria and host plants in which important signaling molecules, e.g. plant flavonoids and rhizobial lipooligosaccharides, play key roles. Here, we introduce a new aspect of this symbiosis: the adaptive response to hosts. more...

Organism:

Bradyrhizobium japonicum; Macroptilium atropurpureum; Glycine max; Bradyrhizobium diazoefficiens USDA 110; Vigna unguiculata subsp. unguiculata

Type:

Expression profiling by array

Platform:

GPL3401

15 Samples

Download data: CEL, CHP

(Submitter supplied) In this study we have looked at the transcriptome profile of both incompatible and compatible cowpea-RKN interaction for two different time points using the Affymetrix soybean GeneChip. This is the first study of this kind in cowpea-RKN interaction. This study provides a broad insight into the Rk-mediated resistance in cowpea and creates an excellent dataset of potential candidate genes involved in both nematode resistance and parasitism, which can be further tested for their role in this biological process using functional genomics approaches. more...

Organism:

Glycine max; Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL4592

28 Samples

Download data: CEL, CHP

(Submitter supplied) Background: Cowpea (Vigna unguiculata L. Walp) is an important food and fodder legume of the semiarid tropics and subtropics worldwide, especially in sub-Saharan Africa. High density genetic linkage maps are needed for marker assisted breeding but are not available for cowpea. A single feature polymorphism (SFP) is a microarray-based marker which can be used for high throughput genotyping and high density mapping. more...

Organism:

Glycine max; Vigna unguiculata

Type:

Expression profiling by array

Platform:

GPL4592

8 Samples

Download data: CEL, CHP

(Submitter supplied) Agilent SurePrint G3 Custom Microarray representing 29,728 unigenes from the cowpea unigene assembly P12.

Organism:

Vigna unguiculata

1 Series

1 Sample

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(Submitter supplied) Arrays of this design have barcodes that begin with 16050749 or 2550749. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Vigna unguiculata

1 Series

12 Samples

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(Submitter supplied) Each probe is replicated 3 times. Ten copies of each of eight control probes derived from the yeast genome sequence with no matches to the unigene sequences were also included in the microarray. A custom Roche NimbleGen microarray was designed based on 29,728 unigene sequences from assembly P12 of cowpea cDNAs in the HarvEST database (http://harvest.ucr.edu/). With the exception of 189 unigenes that failed to pass quality filtering for probe design, each of the unigenes was represented by a set of four 60-mer probes on the fabricated microarray. more...

Organism:

Vigna unguiculata

1 Series

24 Samples

Download data: NDF, NGD

(Submitter supplied) A 385,000 feature microarray was fabricated (Nimblegen Inc., Madison, WI) using 60-nucleotide long oligonucleotide synthesized based upon the nucleotide sequence of 43,253 cowpea unigenes of known and unknown function previously identified in a gene-space sequencing study array_description.ndf array_description.ngd Protocol: High-density DNA array prepared via NimbleGen protocol. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Vigna unguiculata

1 Series

12 Samples

Download data: NDF, NGD

(Submitter supplied) 1. PCR fragments of a cloned cDNA SSH library are arrayed in Genetix X7022 384-well plates. 2. Each well contains a final concentration of 125 ng/µl PCR product in 50% DMSO spotting solution (v/v). 3. Between printing runs the plates are stored at -20°C. 4. Prior to printing the plates are incubated at room temperature for 30 min to aid temperature equilibration. 5. Plates are centrifuged at 2000 x g for 5 minutes prior to printing. more...

Organism:

Vigna unguiculata

1 Series

12 Samples

Download data: GAL