GEO DataSets

(Submitter supplied) Dunaliella salina Bardawil (also known as Dunaliella bardawil) is an extremophilic, unicellular green alga from the Chlorophyte lineage. D. salina is found in hypersaline environments where it can tolerate extremes of heat, light, pH, and up to saturating concentrations of salt. The D. salina Bardawil isolate (UTEX LB 2538) was found in a salt pond near the Bardawil Lagoon on the Sinai peninsula in 1976. more...

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33001

6 Samples

Download data: FASTA, GFF3, TSV

(Submitter supplied) The unicellular green alga Dunaliella salina is an attractive model organism for studying photoacclimation responses. Changes in irradiance levels during cell growth affect the organization and structure of the photosystem and the pigments composition. We sequenced the RNA of D. salina to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. more...

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25649

9 Samples

Download data: FASTA, TXT

(Submitter supplied) Light quality is an important abiotic factor that affects growth and development of photosynthetic organism. In this study, D. salina was exposed to red (660 nm) and blue light (450 nm), and cell growth, pigments, and transcriptome were analyzed. The RNA of D. salina was sequenced and transcriptomic response of algal cells after transitioning from white light to red and blue light was investigated. Genes encoding for enzymes involved in photosynthesis were down-regulated, whereas genes involved in the metabolism of carotenoid were up-regulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging were up-regulated under both red and blue light. The present transcriptomic study would assist in the comprehensive understanding of carotenoid biosynthesis of D. salina.

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25649

9 Samples

Download data: FASTA, XLS

(Submitter supplied) Dunaliella salina, a unicellular and eukaryotic alga, has been found as one of the most salt-tolerant eukaryote with a short growth period and wide practical applications. To elucidate the underlying molecular mechanism involved in the response to salinity and its different effects, RNA-seq was used for global transcriptome profiling of D. salina exposed to NaCl, Sorbiol and H2O2 stress. To maintain osmotic pressure homeostasis under suboptimal environment condition, starch breakdown catalyzed by both alpha-amylase (AMY) and glycogen phosphorylase (PYG) with consecutive expression patterns and low activity of PYG at the beginning of salt stress might be caused by a shortage of ATP because of impaired photosynthesis. more...

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24723

12 Samples

Download data: FA, TXT

(Submitter supplied) Illumina HiseqTM2000 RNA-sequencing of Dunaliella salina under nitrogen deprivation condition was performed.

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21086

1 Sample

Download data: FASTA, XLSX

(Submitter supplied) The unicellular microalga Dunaliella salina is one of the halotolerant and cell wall-less green microalgae in Dunaliella genus. The ability of halotolerance in Dunaliella is attributed to the accumulation of glycerol. Both sugar made by photosynthesis and starch serve as carbon sources for glycerol biosynthesis. Quantitative PCR-based analyses concluded no apparent transcriptional regulation of glycerol, carbon fixation, and starch metabolisms upon salinity stresses. more...

Organism:

Dunaliella salina

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21086

18 Samples

Download data: FASTA, TXT

(Submitter supplied) Algae of the genus Dunaliella are known to be able to grow in a wide range of salinities. To investigate which genes may be important for growth under extremely low or high salinities, a survey of the Dunaliella sp. transcriptome was performed by microarray analysis. For the cDNA microarray 778 expressed sequence tags (ESTs) were generated from a cDNA library of Dunaliella sp. The cDNA microarray was used to compare the transcription profile of cells that were acclimated to extreme saline conditions (0.08 M and 4.5 M NaCl) with the transcription profile of cells acclimated to an intermediate salinity at 1.5 M NaCl. more...

Organism:

Dunaliella salina

Type:

Expression profiling by array

Platform:

GPL6089

4 Samples

Download data: TXT

(Submitter supplied) We constructed the cDNA microarrays from the Dunaliella sp. cDNA library (Park et al., 2006). 1052 selected expressed sequence tags (ESTs) from each clone, including housekeeping genes like ribosomal binding proteins, were amplified by PCR using M13 primers and recovered by a MultiScreen PCR Filter Plate (Millipore, Billerica, MA, USA). Each cDNA chip was designed with 778 successfully amplified PCR products from the cDNA library. more...

Organism:

Dunaliella salina

1 Series

4 Samples

Download data: TXT