GEO DataSets

(Submitter supplied) In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. more...

Organism:

Bombyx mori; Heterorhabditis bacteriophora; Xenorhabdus; Photorhabdus; Spodoptera frugiperda; Galleria mellonella; Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL32011

10 Samples

Download data: RCC, TXT

(Submitter supplied) Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. In the entomopathogenic bacterium Photorhabdus luminescens, a dam ortholog was identified. Overexpression of dam in P. luminescens did not impair growth ability in vitro. In contrast, compared to a control strain harboring an empty plasmid, a significant decrease in motility was observed in the dam-overexpressing strain. more...

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26023

6 Samples

Download data: TXT

(Submitter supplied) A phoP mutant of the entomopathogenic bacterium P. luminescens is attenuated in virulence in insects and susceptible to antimicrobial peptides such as Polymyxin B. The first goal of this study is to compare transcriptomes of the phoP mutant and wild type strain to identify the PhoP regulon. (i) We first compared both strains grown in LB medium. (ii) As we know that low Mg conditions induce expression of PhoP-dependent genes, we also compared transcriptomes of the phoP and wild-type strain after growth in M9 low Mg and in M9 High Mg. more...

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21300

12 Samples

Download data: TXT

(Submitter supplied) Photorhabdus luminescens bacteria alternate lifestyles between its pathogenic insect host and its mutualistic nematode host. We found that Photorhabdus changes radically from the insect pathogenic form (P-form) to slow growing small cells when initiating mutualism (M-form). Here we characterize the gene expression of the M-form relative to the P-form.

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

Type:

Expression profiling by array

Platform:

GPL14574

3 Samples

Download data: GPR

(Submitter supplied) Photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. Variants of Photorhabdus frequently arise. VAR* is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis. In this study, we evaluated the role of transcriptomic modulation in determining the phenotypic variation and delayed pathogenicity of VAR* with respect to the corresponding wild-type form, TT01alpha. more...

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

Type:

Expression profiling by array

Platform:

GPL11042

4 Samples

Download data: TXT

(Submitter supplied) http://www.nanostring.com

Organism:

Xenorhabdus; Bombyx mori; Spodoptera frugiperda; Galleria mellonella; Drosophila melanogaster; Photorhabdus; Heterorhabditis bacteriophora

1 Series

10 Samples

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(Submitter supplied) see manufacturer's website

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

1 Series

3 Samples

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(Submitter supplied) spotted PCR amplicons (300-600 bp) Protocol: 1. Primers were designed by use of a modified version of Primer 3 software (CAAT-Box) to amplify specific fragments (300 to 600 bp). 2. Primers were synthetized by Operon (25pmol/µl). The mix of the forward and reverse primers were provided in 96 deepwell plates. 3. Primers were transfered in 384 well plates Thermofast Abgenes (Merck) and mixed with dNTP (25mM), Titanium DNA polymerase (5U/µL; Clontech) and Photorhabdus luminescens TT01 genomic DNA (30 ng/µL). more...

Organism:

Photorhabdus laumondii subsp. laumondii TTO1

1 Series

4 Samples

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