txid114398[Organism:noexp] - GEO DataSets

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Select item 2001938701.

Regulation of chromatin accessibility in early embryos of the common house spider Parasteatoda tepidariorum

(Submitter supplied) We identified a lineage-specific GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the common house spider Parasteatoda tepidariorum. To investigate possible involvement of the early fuchi activity in regulation of chromatin accessibility, we used an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). Whole embryos at stage 3 that were untreated (wild-type) or treated for fuchi or hedgehog (hh) parental RNAi (pRNAi), as well as wild-type whole embryos at stages 1 and 2, were analyzed by ATAC-seq. more...

Organism:: Parasteatoda tepidariorum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL31240
10 Samples

Download data: BW, NARROWPEAK

Series

Accession:: GSE193870

ID:: 200193870

PubMed Full text in PMC Similar studies

Select item 2001936502.

Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) We identified a GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the spider Parasteatoda tepidariorum. fuchi transcript is initially expressed in cells at both polar regions of the embryo and later in endodermal and extraembryonic cells. To genome-widely identify genes whose expressions are regulated by fuchi, we conducted RNA-sequencing of whole embryos at three different early stages (stages 2 and 3 and early stage 5) that were untreated (control) or treated for fuchi parental RNA interference (pRNAi).

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24841
12 Samples

Download data: BW, TXT

Series

Accession:: GSE193650

ID:: 200193650

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Select item 2001935113.

Genome-wide identification of candidate genes whose transcripts are locally expressed in early embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) The early embryo of the spider Parasteatoda tepidariorum initially shows spherical symmetry in morphology. Following a symmetry breaking event, it forms a radially symmetric germ disc in the hemisphere of the egg. In this work, we conducted RNA-sequencing of cells isolated from small different regions of radially symmetric forming/formed germ discs, aming to identify candidate genes whose transcripts are locally expressed in early spider embryos.

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24841
16 Samples

Download data: BW, TXT

Series

Accession:: GSE193511

ID:: 200193511

PubMed Full text in PMC Similar studies

Select item 2002017054.

Single-cell and single-nucleus transcriptome analysis of pattern formation in the early embryo of the spider Parasteatoda tepidariorum

(Submitter supplied) In a cellular field of the early spider embryo, Hedgehog signaling operates to specify a “fuzzy” French-flag-like pattern along the primary axis. We applied single-cell and single-nucleus RNA sequencing to the early spider embryo. We confirmed that these techniques successfully detected three cell population corresponding to germ layers and some known cell types. We showed that the data had sufficient information for reconstruction of a correct global polarity of the presumptive ectoderm.

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL31240
3 Samples

Download data: CSV, TXT

Series

Accession:: GSE201705

ID:: 200201705

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Select item 2001363575.

Genome-wide Identification of genes that transcriptionally respond to altered states of Hedgehog signaling in embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) Hedgehog signaling plays pivotal roles in formation of the embryonic anterior-posterior axis in the spider Parasteatoda tepidariorum. In this work, using the combination of RNA-sequencing and parental RNA interference (pRNAi), we genome-widely identified genes that transcriptionally responded to altered states of Hedgehog signaling in spider embryos, which were caused by Pt-hedgehog (Pt-hh) pRNAi and Pt-patched (Pt-ptc) pRNAi. more...

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24841
75 Samples

Download data: BW, TXT, WIG

Series

Accession:: GSE136357

ID:: 200136357

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001124356.

Identification of genes whose expression is affected by ptc + dpp double RNAi in spider embryos

(Submitter supplied) In the spider Parasteatoda tepidariorum, Hedgehog (Hh) and Decapentaplegic (Dpp) signaling play key roles in the formation of the two major embryonic axes (Akiyama-Oda and Oda, 2010, Development 137, 1263-1273; Akiyama-Oda and Oda, 2006, Development 133, 2347-2357). Patched (Ptc), a receptor for Hh,functions as a negative regulator of Hh signaling. Double parental RNA interference (pRNAi) against spider homologs of ptc and dpp, Pt-ptc and Pt-dpp, respectively, results in severe defects in axis formation. more...

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by array

Platforms:: GPL11391 GPL11390
2 Samples

Download data: JPG, XLS

Series

Accession:: GSE112435

ID:: 200112435

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Select item 2001130647.

Identification of genes whose expression is affected by Delta RNAi in spider embryos

(Submitter supplied) In the spider Parasteatoda tepidariorum embryo, Delta-Notch signaling plays a key role in early development of the opisthosomal region by regulating the balance between the caudal ectoderm and mesoderm fates (Oda et al., 2007, Development 134, 2195-2205). In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Delta-Notch signaling during early phases of the caudal development.

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by array

Platform:: GPL24882
1 Sample

Download data: JPG, XLS

Series

Accession:: GSE113064

ID:: 200113064

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001127128.

Developmental profiling of gene expression in embryos of the spider Parasteatoda tepidariorum

(Submitter supplied) The common house spider Parasteatoda tepidariorum is a chelicerate model organism for studying developmental mechanisms and their evolution in arthropods. In contrast to the well-studied model insect, Drosophila melanogaster, embryos of the spider undergo patterning in a cellular environment from early stages (at least after the number of the nuclei increase to 16). Use of spider embryos provide new opportunities to understand the evolution of developmental mechanisms underlying arthropod body plans. more...

Organism:: Parasteatoda tepidariorum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24841
20 Samples

Download data: TXT, WIG

Series

Accession:: GSE112712

ID:: 200112712

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000264689.

Identification of genes whose expression is affected by hedgehog RNAi in spider embryos

(Submitter supplied) In the spider Achaearanea tepidariorum, Hedgehog (Hh) signaling plays a key role in the formation of the two major embryonic axes. Analyses of expression patterns of the spider hh homolog, At-hh, suggested that Hh signaling might be involved in the subsequent segmentation process also. In this microarray experiment, we attempted to identify candidate genes whose expressions are regulated by Hh signaling during early phases of spider segmentation.