Tortula linguifolia - GEO DataSets

Select item 2001376091.

To study the effect of moss extract as anthelmintic on cestodes and nematodes of chick and sheep

(Submitter supplied) The bryophyte was collected and dried. Aqueous extract was prepared and different concentration of the extracts were treat against Ascardia galli, chicken nematode and Trichostrongylus, sheep nematode. Control was maintained for both. The control and treated samples were further processed to study the gene expression by isolating and sequencing mRNA using Illumina next-gen sequencing.

Organism:: Ascaridia galli; Trichostrongylus colubriformis

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL27474 GPL27493
4 Samples

Download data: FASTA, TXT

Series

Accession:: GSE137609

ID:: 200137609

SRA Run Selector

Select item 2000136802.

Expression Profiling to Identify Novel Desiccation Response Transcripts from Tortula ruralis gametophytes

(Submitter supplied) In this study we have tried to utilize the unique aspects of the T. ruralis response to desiccation and rehydration to design a strategy to identify rehydrins that are of low abundance and perhaps completely novel to the desiccated or rehydration transcriptomes. We have constructed two Subtractive Suppression Hybridization (SSH) libraries (Diatchenko et al., 1996) that are designed to enrich for differentially expressed low-abundance transcripts contained within gametophytic cells either in the slow-dried state (mRNP sequestrated rehydrin transcripts) or cells that have been rapidly dried, rehydrated and sampled at 2h of hydration (rehydrin and recovery transcripts) when the translational change in gene expression is at its peak (Oliver 1991). more...

Organism:: Syntrichia ruralis

Type:: Expression profiling by array

Platform:: GPL7512
8 Samples

Download data: TXT

Series

Accession:: GSE13680

ID:: 200013680

Select item 1000075123.

Tortula ruralis cDNAs from Suppressive Subtractive Hybridization

(Submitter supplied) We established a cDNA microarray containing the inserts (PCR derived fragment) from each of the 768 individual SSH ESTs, with the exception of thirteen that failed to generate a PCR fragment. Twelve of the missing thirteen SSH EST cDNAs were replaced with PCR fragments from previously isolated T. ruralis cDNAs four of which, representing the ribosomal proteins S14, S16, L23 and L15 (Wood et al., 2000, Zeng and Wood 2000), were previously reported to be constitutively expressed and were added to serve as normalization genes. more...