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Items: 1 to 20 of 3012

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1.

Molecular phenology regulating bud dormancy of the Yoshino-cherry tree

(Submitter supplied) We explored seasonal gene expression profiles in the Yoshino cherry trees (Cerasus × yedoensis ‘Somei-yoshino’) at three distinct sites, each situated at varying latitudes in Japan. Our goal was to gather molecular phenology data that could provide insights into mechanism of bud dormancy. The molecular phenology approach, which monitors the seasonal dynamics of global gene expression profiles in leaves and buds under natural conditions, enables the study of physiological responses to seasonal environmental changes at the molecular level.
Organism:
Prunus yedoensis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33868
90 Samples
Download data: TSV
Series
Accession:
GSE246255
ID:
200246255
2.

Effect of PLX4720 on melanoma-associated fibroblasts co-cultured with melanoma cells

(Submitter supplied) The experiment was designed to assess the effect of PLX4720 on the gene expression in human primary melanoma-associated fibroblast co-cultired with 5555 mouse melanoma cells
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL10558
8 Samples
Download data: TXT
Series
Accession:
GSE274333
ID:
200274333
3.

Prunus dulcis response to novel defense elicitor peptides and control of Xylella fastidiosa infections

(Submitter supplied) Xylella fastidiosa is a plant pathogenic bacterium that has been introduced in the European Union (EU), threatening the agricultural economy of relevant Mediterranean crops such as almond (Prunus dulcis). Plant defense elicitor peptides would be promising to manage diseases such as almond leaf scorch but their effect on the host has not been fully studied. In this work, the response of almond plants to the defense elicitor peptide flg22-NH2 was studied in-depth using RNA-seq, confirming the activation of the salicylic acid and abscisic acid pathways. more...
Organism:
Prunus dulcis
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32033
16 Samples
Download data: TXT
Series
Accession:
GSE259385
ID:
200259385
4.

Transcriptomic analysis revealed genes involved in response to cold stress in Prunus sibirica

(Submitter supplied) Through transcriptome sequencing analysis of clones with strong freezing resistance (338) and weak freezing resistance (28) of Prunus sibirica,a total of 6001 significantly differentially expressed genes were obtained. Among them, there are 2070 up-regulated genes and 3,931 down-regulated genes. Differential genes are compared and screened, and WRKY, NAC, bHLH, MADS, MYB, Homeobox, F-box, ZEP, SBT, ERF, CAMTA, MDH, etc. more...
Organism:
Prunus sibirica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30412
6 Samples
Download data: XLS
Series
Accession:
GSE180451
ID:
200180451
5.

Peach flower bud development

(Submitter supplied) Thinning is indispensable practice in peach cultivation aiming to reduce fruit number per plant, promoting sink-source balance and reducing competition among fruit, which results in bigger fruit and the improvement of other fruit-quality parameters. Inhibition of floral induction by GAs has been largely demonstrated and commercial products based on GAs have been used to this aim. We tested a product GA4/7 based in different moments after full bloom in peach to reduce the number of flowers in the following season. more...
Organism:
Prunus persica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32039
18 Samples
Download data: TXT
Series
Accession:
GSE247681
ID:
200247681
6.

Transcriptome analysis revealed the genes involved in cold stress during flowering from Prunus sibirica

(Submitter supplied) Through transcriptome sequencing analysis of clones with strong freezing resistance (CR_453) and weak freezing resistance (NC_371) of Prunus sibirica,a total of 15,228significantly differentially expressed genes were obtained. Among them, there are 6,630 up-regulated genes and 8,598 down-regulated genes. Differential genes are compared and screened, and B3, MYB, AAA, zf-GRF, bHLH, TCP, WD40, HSP, PPR, Zim, AP2, CBF, etc, Freeze-related gene family.It is also found that a large number of gene families are regulated by hormones, including GST_N、Auxin_inducible, Abhydrolase_1,P450,AP2,ADH_N,Zim,HATPase_c,2OG-FeII_Oxy,IPPT,C2,RRM_1and so on.
Organism:
Prunus sibirica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30412
30 Samples
Download data: TXT
Series
Accession:
GSE204685
ID:
200204685
7.

Transcriptome profiling of Pseudomonas syringae pv. syringae 9644 in rich media and hrp-inducing minimum media

(Submitter supplied) Pseudomonas syringae pv. syringae 9644 (Pss9644) is a causal agent of bacterial cherry canker causing necrotic symptoms on leaves, fruits, gummosis and canker in woody tissues of sweet cherry (Prunus avium). To understand which virulent factor genes were expressed in vitro, Pss9644 was grown in rich media (King's B Broth) and minimum media (hrp-inducing minimum media). The latter mimics the in planta environment.
Organism:
Pseudomonas syringae pv. syringae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL34164
6 Samples
Download data: TXT
Series
Accession:
GSE255102
ID:
200255102
8.

Recognition of copy-back defective interfering rabies virus genomes by RIG-I triggers efficient immune response against vaccine strains

(Submitter supplied) Using next-generation sequencing (NGS) combined with bioinformatics tools, we characterized two major 5’copy-back defective interfering (5’cb DI) genomes generated during SAD replication. Furthermore, we identified a specific interaction of 5’cb DI genomes and RIG-I that correlated with a high stimulation of the type I IFN signaling
Organism:
Lyssavirus rabies; Rabies virus SAD B19; Homo sapiens
Type:
Expression profiling by high throughput sequencing; Other
Platforms:
GPL31906 GPL31905
36 Samples
Download data: CSV
Series
Accession:
GSE196051
ID:
200196051
9.

A phased genome of the highly heterozygous 'Texas' almond uncovers patterns of allele-specific expression linked to heterozygous transposon insertions

(Submitter supplied) The vast majority of traditional almond varieties are self-incompatible and the level of variability of the species is very high, resulting in a highly heterozygosity genome. Therefore, information on the different haplotypes is particularly relevant to understand the genetic basis of trait variability in this species. However, although reference genomes for several almond varieties exist, none of them is phased and has genome information at the haplotype level. more...
Organism:
Prunus dulcis; Prunus persica
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL21993 GPL27530
12 Samples
Download data: FASTA, TXT
Series
Accession:
GSE254853
ID:
200254853
10.

euAP2a, a Key Gene that Regulates Flowering Time with a Novel Mechanism in Peach (Prunus persica)

(Submitter supplied) Spring frost is a growing risk to temperate fruit production as warmer winter conditions can lead to earlier bloom, increasing the chance of damaging cold temperatures. One strategy to minimize the impacts of frost is to breed late-flowering cultivars to avoid the frost risk period. In this study, we analyzed Late-Flowering Peach (LFP) germplasm and showed its floral buds require longer chilling and warming periods during dormancy than the control cultivar, ‘John Boy’ (JB). more...
Organism:
Prunus persica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL25275
84 Samples
Download data: CSV
Series
Accession:
GSE244570
ID:
200244570
11.

Defying Gravity: WEEP promotes negative gravitropism in Prunus persica by establishing asymmetric auxin gradients

(Submitter supplied) Trees with weeping shoot architectures are valued for their beauty and are a resource for understanding how plants regulate posture control. The Prunus persica (peach) weeping phenotype, which has elliptical downward arching branches, is caused by a homozygous mutation in the WEEP gene. Until now, little was known about the function of WEEP protein despite its high conservation throughout Plantae. Here, we present the results of anatomical, biochemical, biomechanical, physiological, and molecular experiments that provide insight into WEEP function. more...
Organism:
Prunus persica
Type:
Expression profiling by high throughput sequencing
Platform:
GPL28214
32 Samples
Download data: XLSX
Series
Accession:
GSE250141
ID:
200250141
12.

Transcriptomic analysis of resistant and wild-type Botrytis ci-nerea isolates revealed fludioxonil-resistant mechanisms

(Submitter supplied) Botrytis cinerea (gray mold) is one of the most destructive pathogens of cherry tomatoes, causing fruit decay and economic loss. Fludioxonil is an effective fungicide widely used for crop protec-tion and is essential for controlling tomato gray mold. The emergence of fungicide-resistant strains has made the control of Botrytis cinerea more difficult. While the genome of Botrytis cinerea is available, there are few reports regarding the large-scale functional annotation of the genome using expressed genes derived from transcriptomes, and the mechanism(s) underlying such flu-dioxonil resistance remain unclear. more...
Organism:
Botrytis cinerea
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32786
18 Samples
Download data: TXT
Series
Accession:
GSE221721
ID:
200221721
13.

Structured wound angiogenesis instructs mesenchymal barrier compartments in the regenerating nerve

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL19057 GPL24247
25 Samples
Download data: MTX, TSV
Series
Accession:
GSE233360
ID:
200233360
14.

Structured wound angiogenesis instructs mesenchymal barrier compartments in the regenerating nerve [bulkRNASeq]

(Submitter supplied) Organ injury stimulates the formation of new capillaries to restore blood supply raising questions about the potential contribution of neoangiogenic vessel architecture to the healing process. With single-cell mapping we resolved the properties of endothelial cells that organize a polarized scaffold at the repair site of lesioned peripheral nerves. Transient reactivation of an embryonic guidance program is required to orient neovessels across the wound. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19057
20 Samples
Download data: TSV
Series
Accession:
GSE233355
ID:
200233355
15.

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Arabidopsis thaliana
Type:
Methylation profiling by high throughput sequencing; Other; Expression profiling by high throughput sequencing
Platforms:
GPL21785 GPL24270 GPL26208
361 Samples
Download data
Series
Accession:
GSE192611
ID:
200192611
16.

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RNA-seq]

(Submitter supplied) In sexually propagating organisms, genetic and epigenetic mutations are evolutionarily relevant only if they occur in the germline and provide inherited information to the next generation. In contrast to most animals, plants are thought to lack an early segregating germline, implying that somatic cells can contribute genetic information to the progeny if they differentiate into meiocytes. Here we demonstrate that two ARGONAUTE proteins, AGO5 and AGO9, are expressed in the reproductive lineage throughout development and mark an early-segregating germline. more...
Organism:
Arabidopsis thaliana
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL24270 GPL26208
67 Samples
Download data: CSV
Series
Accession:
GSE192608
ID:
200192608
17.

Detection of plant microRNAs in edible-plant based products

(Submitter supplied) Plant-based diets could be a key source of microRNAs in animals. Plant microRNAs are cross-kingdom gene expression regulators that could modulate mammalian gene expression, influencing their physiology. Therefore, it is important to identify the microRNA expression profile of plant foods in order to identify potential target genes and biological functions in the mammalian host. Next-generation sequencing was applied to identify microRNAs in RNA samples derived from nuts (walnut and almond), vegetables (spinach) and fruits (orange, apple, olive, pear, and tomato). more...
Organism:
Solanum lycopersicum; Olea europaea; Pyrus communis; Citrus sinensis; Prunus dulcis; Juglans regia; Spinacia oleracea; Malus domestica
Type:
Non-coding RNA profiling by high throughput sequencing
8 related Platforms
8 Samples
Download data: XLSX
Series
Accession:
GSE234786
ID:
200234786
18.

Efficient genome editing in erythroid cells unveils novel MYB target genes and regulatory functions

(Submitter supplied) Targeted genome editing holds great promise in biology. However, efficient genome modification, including gene knock-in, remains an unattained goal in multiple cell types and loci due to poor transfection efficiencies and low target genes expression, impeding the positive selection of recombined cells. Here, we describe a genome editing approach to achieve efficient gene targeting using hard to transfect erythroid cell lines. more...
Organism:
Mus musculus
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL21103
4 Samples
Download data: BED
Series
Accession:
GSE228372
ID:
200228372
19.

Defining the Sox2-independent reprogramming steps to pluripotency

(Submitter supplied) Several Sox family members, small molecules, and inhibitors of the TGF-β pathway can replace exogenous Sox2 during the generation of induced pluripotent stem cells (iPSCs), suggesting that SOX2 is dispensable for initiation of reprogramming. However, the time point at which the endogenous Sox2 locus is activated in these conditions was not thoroughly evaluated. To identify the Sox2-independent reprogramming steps, we excluded Sox2 from the reprogramming cocktail and used Sox2-deficient mouse embryonic fibroblasts. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL6885
16 Samples
Download data: TXT
Series
Accession:
GSE59372
ID:
200059372
20.

Expression patterns of white blood cells in response to individual food treatments relative to LPS treatment and untreated in an FPIES subject

(Submitter supplied) Total blood white blood cells from two FPIES subjects were treated with 2 foods that were safe for those subjects, 3 foods that were triggers for those subjects, relative to LPS and untreated for each subject
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
9 Samples
Download data: MTX, TSV
Series
Accession:
GSE232839
ID:
200232839
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