(Submitter supplied) This study describes the distribution and functional analysis of ATP-dependent chromatin remodelers in mouse 46C ES cells. The remodelers for which ChIP-Seq profiles were generated are Brg1, Chd1, Chd2, Chd4, Chd6, Chd8, Chd9 and Ep400. We first generated ES cell lines expressing individual remodelers fused to an affinity tag at the C-terminus, from their endogenous loci. Remodelers were then formaldehyde-crosslinked to chromatin in vivo, MNase digested to release individual nucleosomes, then immunoprecipitated sequentially with two distinct antibodies against the tag.
more...- Organism:
- Mus musculus
- Type:
- Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing
- 5 related Platforms
- 44 Samples
Download data: BEDGRAPH, BW, WIG