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Items: 6

1.

Immunoprecipitation of PTB65 associated mRNAs

(Submitter supplied) PTB is multifunctional RNA binding protein reported to be involved in splicing, 3' -end processing, stability and translational regulation. Sample preparation methods (Gama-Carvalho et al, 2006, submitted) Suspension HeLa cells were grown in DMEM, 10% FCS, Pen/Strep and split 1:2 the day before harvesting. Post-nuclear cytoplasmic extracts were obtained as described (Herbert and Hecht 1999), using 5x107 cells/ml of lysis buffer. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
4 Samples
Download data: CEL
Series
Accession:
GSE6021
ID:
200006021
2.

[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html June 03, 2009: annotation table updated with netaffx build 28 June 06, 2012: annotation table updated with netaffx build 32 June 23, 2016: annotation table updated with netaffx build 35 Protocol: see manufacturer's web site Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. more...
Organism:
Homo sapiens
602 DataSets
5645 Series
61 Related Platforms
171173 Samples
Download data
Platform
Accession:
GPL570
ID:
100000570
3.

Immunoprecipitated cDNA HeLa cell extract from Immunoprecipitation experiment 2 PTB

Organism:
Homo sapiens
Source name:
anti-PTB immunoprecipitate from HeLa cell cytoplasmic extract
Platform:
GPL570
Series:
GSE6021
Download data: CEL
Sample
Accession:
GSM139835
ID:
300139835
4.

Total cDNA from HeLa cell extract from Immunoprecipitation experiment 2 PTB

Organism:
Homo sapiens
Source name:
HeLa cell cytoplasmic extract
Platform:
GPL570
Series:
GSE6021
Download data: CEL
Sample
Accession:
GSM139834
ID:
300139834
5.

Immunoprecipitated cDNA HeLa cell extract from Immunoprecipitation experiment 1 PTB

Organism:
Homo sapiens
Source name:
anti-PTB immunoprecipitate from HeLa cell cytoplasmic extract
Platform:
GPL570
Series:
GSE6021
Download data: CEL
Sample
Accession:
GSM139833
ID:
300139833
6.

Total cDNA from HeLa cell extract from Immunoprecipitation experiment 1 PTB

Organism:
Homo sapiens
Source name:
HeLa cell cytoplasmic extract
Platform:
GPL570
Series:
GSE6021
Download data: CEL
Sample
Accession:
GSM139832
ID:
300139832
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Supplemental Content

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