GSE158013[Accession] - GEO DataSets - NCBI

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Select item 2001580131.

Simultaneous trimodal single cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq

(Submitter supplied) Single-cell measurements of cellular characteristics have been instrumental in understanding the heterogeneous pathways that drive differentiation, cellular responses to signals, and human disease. Recent advances have allowed paired capture of protein abundance and transcriptomic state, but a lack of epigenetic information in these assays has left a missing link to gene regulation. Using the heterogeneous mixture of cells in human peripheral blood as a test case, we developed a novel scATAC-seq workflow that increases signal-to-noise and allows paired measurement of cell surface markers and chromatin accessibility: Integrated Cellular Indexing of Chromatin Landscape and Epitopes (ICICLE-seq). more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:: GPL24676 GPL18573
34 Samples

Download data: CSV, H5, TSV

Series

Accession:: GSE158013

ID:: 200158013

PubMed Full text in PMC Similar studies

Select item 1000246762.

Illumina NovaSeq 6000 (Homo sapiens)

Organism:: Homo sapiens

14305 Series
873861 Samples

Platform

Accession:: GPL24676

ID:: 100024676

Select item 1000185733.

Illumina NextSeq 500 (Homo sapiens)

Organism:: Homo sapiens

11938 Series
329075 Samples

Platform

Accession:: GPL18573

ID:: 100018573

Select item 3051239544.

TEA-seq of leukapheresis-purified, 0.01% digitonin perm, FACS neutrophil-depleted PBMCs, method comparison, well 4

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123954

ID:: 305123954

Select item 3051239535.

TEA-seq of leukapheresis-purified, 0.01% digitonin perm, FACS neutrophil-depleted PBMCs, method comparison, well 3

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123953

ID:: 305123953

Select item 3051239526.

TEA-seq of leukapheresis-purified, 0.01% digitonin perm, FACS neutrophil-depleted PBMCs, method comparison, well 2

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123952

ID:: 305123952

Select item 3051239517.

TEA-seq of leukapheresis-purified, 0.01% digitonin perm, FACS neutrophil-depleted PBMCs, method comparison, well 1

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123951

ID:: 305123951

Select item 3051239508.

Multiome RNA/ATAC of leukapheresis-purified, 0.01% digitonin perm, FACS neutrophil-depleted PBMCs, method comparison

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123950

ID:: 305123950

Select item 3051239499.

Multiome RNA/ATAC of leukapheresis-purified, isolated nuclei, FACS neutrophil-depleted PBMCs, method comparison

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM5123949

ID:: 305123949

Select item 30494991110.

TEA-seq of leukapheresis-purified, 0.01% digitonin permeabilized, FACS neutrophil-depleted PBMCs, well 1

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4949911

ID:: 304949911

Select item 30478408811.

ICICLE-seq of leukapheresis-purified, 0.01% digitonin permeabilized PBMCs, well 3

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784088

ID:: 304784088

Select item 30478408712.

ICICLE-seq of leukapheresis-purified, 0.01% digitonin permeabilized PBMCs, well 2

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784087

ID:: 304784087

Select item 30478408613.

ICICLE-seq of leukapheresis-purified, 0.01% digitonin permeabilized PBMCs, well 1

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784086

ID:: 304784086

Select item 30478408514.

scATAC-seq of ficoll-purified, 0.01% digitonin permeabilized, FACS neutrophil-depleted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784085

ID:: 304784085

Select item 30478408415.

scATAC-seq of ficoll-purified, 0.01% digitonin permeabilized, FACS dead cell/debris-depleted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784084

ID:: 304784084

Select item 30478408316.

scATAC-seq of ficoll-purified, 0.01% digitonin permeabilized, unsorted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784083

ID:: 304784083

Select item 30478408217.

scATAC-seq of ficoll-purified, 1x 10xNIB isolated nuclei, FACS neutrophil-depleted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784082

ID:: 304784082

Select item 30478408118.

scATAC-seq of ficoll-purified, 1x 10xNIB isolated nuclei, FACS dead cell/debris-depleted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784081

ID:: 304784081

Select item 30478408019.

scATAC-seq of ficoll-purified, 1x 10xNIB isolated nuclei, unsorted PBMCs from donor RG1131

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784080

ID:: 304784080

Select item 30478407920.

scATAC-seq of leukapheresis-purified, 0.01% digitonin permeabilized, magnetic bead anti-CD15-depleted PBMCs

Organism:: Homo sapiens

Source name:: peripheral blood mononuclear cells

Platform:: GPL24676
Series:: GSE158013

Download data: CSV, H5, TSV

Sample

Accession:: GSM4784079

ID:: 304784079

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