GEO DataSets

(Submitter supplied) We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes.

Organism:

Drosophila montana

Type:

Expression profiling by array

Platform:

GPL9293

9 Samples

Download data: TXT

(Submitter supplied) The primers for the candidate genes of D. montana were designed for one to three unique 200-500 bp regions with Primer3 program (http://www-genome.wi.mit.edu/genome software/other/ primer3.html) or manually, using information on the gene alignments mentioned above. Next, an online program e-Array 5.3 (Agilent) was used to design one to four 60 bp oligonucleotide probes per gene using exon regions. Finally, the probes were synthesized in situ with liquid chemistry and arrayed on an “Agilent 60-mer Multi-Pack Gene Expression Microarray” platform with one-colour system. more...

Organism:

Drosophila montana

1 Series

9 Samples

Download data