GPL2675[Accession] - GEO DataSets

Select item 2000053431.

Small RNA identification in Arabidopsis thaliana using 454 data

(Submitter supplied) The Arabidopsis genome contains a highly complex and abundant population of small RNAs, and many of the endogenous siRNAs are dependent on RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) for their biogenesis. By analyzing an rdr2 loss-of-function mutant using two different parallel sequencing technologies, MPSS and 454, we characterized the complement of miRNAs expressed in Arabidopsis inflorescence to considerable depth. more...

Organism:: Arabidopsis thaliana

Type:: Expression profiling by high throughput sequencing; Expression profiling by MPSS

Platforms:: GPL2675 GPL4011
6 Samples

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Series

Accession:: GSE5343

ID:: 200005343

Select item 2000030082.

Small RNA identification in Arabidopsis thaliana using modified MPSS

(Submitter supplied) Small RNAs play important regulatory roles in most eukaryotes but only a small proportion of these molecules have been identified. We sequenced more than two million small RNAs from seedlings and the inflorescence of the model plant Arabidopsis thaliana. Known and new miRNAs were among the most abundant of the non-redundant set of more than 75,000 sequences, whereas more than half represented lower abundance small-interfering RNAs (siRNAs) that match repetitive sequences, intergenic regions, and genes. more...

Organism:: Arabidopsis thaliana

Type:: Expression profiling by MPSS

Platform:: GPL2675
4 Samples

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Series

Accession:: GSE3008

ID:: 200003008

Select item 1000026753.

Small RNA identification in Arabidopsis thaliana using modified MPSS

(Submitter supplied) All MPSS was performed essentially as described by Brenner et al. (Nat Biotechnol. 2000 18:630). The specific modifications to the method for the MPSS sequencing of small RNAs are described in the paper associated with this dataset (Lu et al., 2005). The signatures with "flag = 1" match to tRNAs, rRNAs, snoRNAs and snRNAs.