Table 4:

Results from Example 2: to determine the capture and detection antibody concentrations. Values are averages of absorbance measurements at A450.

Capture Antibody2 µg/ml1 µg/ml0.5 µg/ml
Analyte protein ng/ml501050105010
Detection Antibody 1:10003.691.811.373.631.891.333.31.790.99
1:50003.220.70.493.240.810.473.10.830.36
1:250001.610.210.151.750.250.161.720.260.12

From: Immunoassay Methods

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Assay Guidance Manual [Internet].
Markossian S, Grossman A, Arkin M, et al., editors.
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