| Compound Information | Screening Assays | Probe Development Assays (AID-2088) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Compound | Structure | CID | SID | MLS | Vendor | Synthesized at the SRIMSC | Wee1 Primary (AID-1321) | Wee1 Confirmation (AID-1410) | Wee1 Dose Response (AID-1412) | Cytotoxicity Assay (CC50) (AID-1413) | Cyclin B EC50 (AID-1414) | SID | Wee1 MAX % ACT | Wee1 EC50 | Cyclin B MAX % ACT | Cyclin B EC50 (uM) | G2/M Arrest Assay |
| Compound 1 |
 | 690911 | 4256064 | MLS 000061388 | Chem Bridge | No | Active | Active | Active (1.842 μM) | Inactive (49.75 μM) | Inactive (49.75 μM) | 87235991 | 186.39 | 6.19 | 47.27 | Inactive (>55.7) | Not tested due to lower Wee1 selectivity compared to the probe. |
| Compound 2 |
 | 755456 | 56432189 | None | TSRI | Yes | These compounds were not in the MLSMR collection. | 56432189 | 3.75 | Inactive | 14.7 | Inactive | Not tested due to lower % activation of Wee1, compared to the probe. | ||||
| Compound 3 |
 | 2837745 | 56431998 | None | TSRI | Yes | 56431998 | 6.78 | Inactive | 8.09 | Inactive | Not tested due to lower Wee1 selectivity compared to the probe. | |||||
From: Small Molecule Inhibitors of Wee1 Degradation and Mitotic Entry
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