Table 2Reagents used for the uHTS experiments

ReagentVendor
Human Embryonic Kidney Cells stably transduced with a 5X NF-κB RE (response elements) upstream of a firefly luciferase cassetteCell stocks from AP, scale up by BCCG
Ala-γ-Glu-diaminopimelic acid – inducer of NOD1 pathway to activation of NF-κBAna Spec Cat #60774 (initial lot donated by AP)
Commercial lumigenic Luciferase substrate $1,992.00/L (Britelite™)Perkin-Elmer

From: High Throughput Screening Assays for NOD1 Inhibitors - Probe 2

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Probe Reports from the NIH Molecular Libraries Program [Internet].
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