| PubChemBioAssay Name | AID | Probe Type | Assay Type | Assay Format | Assay Detection & well format |
|---|---|---|---|---|---|
| Summary assay for the identification of compounds that inhibit NOD1 [Summary] | 1575 | Inhibitor | Summary | N/A | N/A |
| uHTS luminescence assay for the identification of compounds that inhibit NOD1 [Confirmatory] | 1578 | Inhibitor | Primary | Cell- based | luminescence & 1536 |
| uHTS luminescence assay for the identification of compounds that inhibit NOD2 [Confirmatory] | 1566 | Inhibitor | Counterscreen for NOD1 (also NOD2 Primary) | Cell- based | luminescence & 1536 |
| HTS assay for identification of inhibitors of TNFα-specific NF-κB induction | 1852 | Inhibitor | Counterscreen | Cell- based | luminescence & 1536 |
| uHTS Fluorescence assay for the identification of cytotoxic compounds among compounds active in NOD1 cell inhibition assay [Confirmatory] | 1849 | Inhibitor | Cytotoxicity Counterscreen | Cell- based | luminescence & 1536 |
| uHTS luminescence assay for the identification of compounds that inhibit NOD2 in MDP treated cells. [Confirmatory] | 2001 | Inhibitor | Counterscreen | Cell- based | luminescence & 1536 |
| SAR analysis of compounds that inhibit NOD1 [Confirmatory] | 2333 | Inhibitor | SAR | Cell- based | Luminescence & 384 |
| SAR analysis of compounds that inhibit NOD1 – Set 2 [Confirmatory] | 2466 | Inhibitor | SAR | Cell- based | Luminescence & 384 |
| SAR analysis of compounds that inhibit NOD2 [Confirmatory] | 2334 | Inhibitor | SAR | Cell- based | Luminescence & 384 |
| SAR analysis of compounds that inhibit NOD2 – Set 2 [Confirmatory] | 2475 | Inhibitor | SAR | Cell- based | Luminescence & 384 |
| SAR analysis of inhibitors of TNFα specific NF- κB induction [Confirmatory] | 2337 | Inhibitor | SAR Counterscreen | Cell- based | Luminescence & 1536 |
| SAR analysis of inhibitors of TNFα specific NF- κB induction – Set 2 [Confirmatory] | 2483 | Inhibitor | SAR Counterscreen | Cell- based | Luminescence & 1536 |
| SAR analysis of compounds that are cytotoxic to HEK293 [Confirmatory] | 2335 | Inhibitor | SAR Cytotoxicity Counterscreen | Cell- based | luminescence & 1536 |
| SAR analysis of compounds that are cytotoxic to HEK293 – Set 2 [Confirmatory] | 2335 | Inhibitor | SAR Cytotoxicity Counterscreen | Cell- based | luminescence & 1536 |
| SAR analysis of muramyl dipeptide (MDP) induced IL-8 secretion in MCF-7/NOD2 cells.– Set 2 [Confirmatory]] | 2503 | Inhibitor | Secondary Assay for specificity | Cell- based | Absorbance (at 450 nm) & 96 |
| SAR analysis of tumor necrosis factor alpha (TNFαlpha) induced IL-8 secretion in MCF- 7/NOD1 cells – Set 2 [Confirmatory] | 2504 | Inhibitor | Secondary Assay for specificity | Cell- based | Absorbance (ELISA) of cell extracts & 96 |
| SAR analysis of GM-Tri-DAP induced IL-8 secretion in MCF-7/NOD1 cells – Set 2 [Confirmatory] | 2505 | Inhibitor | Secondary Assay for specificity | Cell- based | Absorbance (ELISA) of cell extracts & 96 |
| SAR analysis of NF-κB dependent luciferase using DAP as an inducer – Set 2 [Confirmatory] | 2793 | Inhibitor | Secondary Assay for specificity | Cell- based | luminescence & 96 |
| SAR analysis of NF-κB dependent luciferase using PMA/Ionomycin as an inducer – Set 2 [Confirmatory] | 2792 | Inhibitor | Secondary Assay for specificity | Cell- based | luminescence & 96 |
| SAR analysis of NF-κB dependent luciferase using Doxorucibin as an inducer – Set 2 [Confirmatory] | 2789 | Inhibitor | Secondary Assay for specificity | Cell- based | luminescence & 96 |
From: High Throughput Screening Assays for NOD1 Inhibitors - Probe 2
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