Table 2:

Endonucleases with different PAM specificity.

NameOrganismPAMTypeRef
SpCas9 Streptococcus pyogenes 5’-NGG-3’II(11)
SpCas9 VQR Streptococcus pyogenes 5’-NGAN-3’II(105,106)
SpCas9 EQR Streptococcus pyogenes 5’-NGNG-3’II(105,106)
SpCas9 VRER Streptococcus pyogenes 5’-NGCG-3’II(105,106)
SaCas9 Staphylococcus aureus 5’-NNGRRT-3’II(105,107)
NmCas9 Neisseria meningitides 5’-NNNNGATT-3’II(108)
St1Cas9 Streptococcus thermophiles 5’-NNAGAAW-3’II(109)
St3Cas9 Streptococcus thermophiles 5’-NNGGNG-3’II(109)
BlCas9 Brevibacillus laterosporus 5’-NNNCND-3’II(110)
AsCpf1
LbCpf1		 Acidaminococcus
Lachnospiraceae 		5’-TTTC-3’V(14)
C2c1 A. acidoterrestris 5’-TTC-3’V(111)

From: Genome Editing Using Engineered Nucleases and Their Use in Genomic Screening

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