TABLE 42

PCR master mix for 13-plex (Big 11)

IngredientStock concentrationFinal concentrationAdd (µl) per reactionAdd (µl) per 96-well plate
PCR water4.7470
MassCode PCR master mix (Agilent)2 ×1 ×101000
MassCode primers3.84615 µM0.25 µM each1.3 µl (13-plex primer mix)130
Total161600

From: Appendix 1, Laboratory standard operating procedures for MassCode polymerase chain reaction for enteric samples

Cover of Can rapid integrated polymerase chain reaction-based diagnostics for gastrointestinal pathogens improve routine hospital infection control practice? A diagnostic study
Can rapid integrated polymerase chain reaction-based diagnostics for gastrointestinal pathogens improve routine hospital infection control practice? A diagnostic study.
Health Technology Assessment, No. 18.53.
Pankhurst L, Macfarlane-Smith L, Buchanan J, et al.
Southampton (UK): NIHR Journals Library; 2014 Aug.
Copyright © Queen’s Printer and Controller of HMSO 2014. This work was produced by Pankhurst et al. under the terms of a commissioning contract issued by the Secretary of State for Health. This issue may be freely reproduced for the purposes of private research and study and extracts (or indeed, the full report) may be included in professional journals provided that suitable acknowledgement is made and the reproduction is not associated with any form of advertising. Applications for commercial reproduction should be addressed to: NIHR Journals Library, National Institute for Health Research, Evaluation, Trials and Studies Coordinating Centre, Alpha House, University of Southampton Science Park, Southampton SO16 7NS, UK.

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