| Nutrient | Unit | Amount, per kg diet | Comment/Reference |
|---|---|---|---|
| Lipid | g | 50.0 | |
| Linoleic acid | g | 6.8 | |
| Protein (N × 6.25) | |||
| Growth | g | 180.0 | Equivalent to 20% casein supplemented with 0.3% DL-methionine or 24% casein |
| g | 200.0 | Casein (see text) | |
| Reproduction | g | 180.0 | Natural-ingredients |
| Amino acids | |||
| Arginine | g | 3.0 | See text |
| Histidine | g | 2.0 | See text |
| Isoleucine | g | 4.0 | See text |
| Leucine | g | 7.0 | See text |
| Valine | g | 5.0 | See text |
| Threonine | g | 4.0 | See text |
| Lysine | g | 4.0 | See text |
| Methionine | g | 5.0 | Cystine may replace 50-66.6% |
| Phenylalanine | g | 7.6 | Tyrosine may replace 50% |
| Tryptophan | g | 1.0 | Niacin may replace 0.025% (see text) |
| Minerals | |||
| Calcium | g | 5.0 | |
| Chloride | g | 0.5 | |
| Magnesium | g | 0.5 | 0.7 g/kg for lactation (see text) |
| Phosphorus | g | 3.0 | |
| Potassium | g | 2.0 | Higher concentrations may be required for lactation (see text) |
| Sodium | g | 0.5 | |
| Copper | mg | 6.0 | 8.0 mg/kg for pregnancy and lactation |
| Iron | mg | 35.0 | |
| Manganese | mg | 10.0 | |
| Zinc | mg | 10.0 | 30 mg/kg for reproduction and lactation |
| Iodine | µg | 150.0 | |
| Molybdenum | µg | 150.0 | |
| Selenium | µg | 150.0 | Selenite form of Se |
| Vitamins | |||
| A (retinol)a | mg | 0.72 | Santhanam et al., 1987 |
| D (cholecalciferol)b | mg | 0.025 | Adequate; no quantitative data |
| E (RRR-α-tocopherol)c | mg | 22.0 | Yasunaga et al., 1982 |
| K (phylloquinone) | mg | 1.0 | Based on the requirement for the rat; Kindberg and Suttie, 1989 |
| Biotin (d-biotin) | mg | 0.2 | Adequate; Fenton et al., 1950 |
| Choline (choline bitartrate) | mg | 2,000.0 | Adequate; insufficient data to establish requirement |
| Folic acid | mg | 0.5 | Fenton et al., 1950; Heid et al., 1992 |
| Niacin (nicotinic acid) | mg | 15.0 | Based on the requirement for the rat; Hundley, 1949 |
| Pantothenate (Ca) | mg | 16.0 | Morris and Lippincott, 1941, suggest 36 mg/kg for reproduction and lactation |
| Riboflavin | mg | 7.0 | Adequate; insufficient data to establish requirement |
| Thiamin (thiamin-HCl) | mg | 5.0 | |
| B6 (pyridoxine-HCl) | mg | 8.0 | 1 mg/kg for maintenance |
| B12 | µg | 10.0 | Adequate; insufficient data to establish requirement |
NOTE: Nutrient requirements are expressed on an as-fed basis for diets containing 10% moisture and 3.8 to 4.1 kcal ME/g (16–17 kJ ME/g) and should be adjusted for diets of differing moisture and energy concentrations. Unless otherwise specified, the listed nutrient concentrations represent minimal requirements and do not include a margin of safety. Higher concentrations for many nutrients may be warranted in natural-ingredient diets.
Equivalent to 2,400 IU/kg of diet.
Equivalent to 1,000 IU/kg of diet.
Equivalent to 32 IU/kg.
From: 3, Nutrient Requirements of the Mouse
NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.