| Entry | Pubchem SID | PBS Solubility (μg/mL)a | Assay Media Solubility (μg/mL)b | Conc. in Assay Media (μM)c | Assay Media Solubility (μM)d | CIT2 EC50 μMe (% resp)f | AGP1 EC50 μM (% resp) | MEP2 EC50 μM (% resp) | LAP4 EC50 μM (% resp) | RPL19A EC50 μM (% resp) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 99300522 (ML231) | 0.05 | 0.07 | 0.24 | 4–6 | 3.8 (91.7%) | > 100 (6.7%) | > 100 (16.0%) | > 100 (12.2%) | > 100 (18.4%) |
| 2 | 96099781 | 2.1 | 4.1 | 14.74 | 10–20 | 3.36 (94.7%) | 11.33 (39.2%) | 12.68 (77.2%) | 9.06 (59.1%) | 6.36 (69.9%) |
| 3 | 110923093 | NA | 0.99 | 3.21 | 4–10 | 0.77 (95.5%) | 0.72 (30.5%) | 0.68 (24.6%) | > 100 (18.2%) | 0.51 (64.9%) |
| 4 | 99300541 | 3.2 | 6.1 | 23.43 | NA | 8.82 (97.7%) | > 100 (5.8%) | > 100 (4.4%) | > 100 (8.1%) | > 100 (18.2%) |
Conditions: 1× PBS buffer, pH 7.4; Data collected by Sanford-Burnham
Conditions: SCD medium with 0.03% Pluronic F-6; Data collected by Sanford-Burnham
Concentration calc’d from assay media solubilityb data from Sanford Burnham and compound mol. wt
Conditions as in footnote b; Data collected by UNMCMD using Flow Cytometry, range shown
EC50 reported as greater than 100 for inactive compound. The actual EC50 for inactive compounds is not shown.
maximum % response compared to rapamycin. Compound scored inactive if % response was < 20%.
NA = data not available
From: Profiling a Selective Probe for RTG Branch of Yeast TORC1 Signaling Pathway
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