|
| Status |
Public on Jul 24, 2012 |
| Title |
clonal adipose cell line from interscapular depot [BAT6] |
| Sample type |
RNA |
| |
|
| Source name |
clonal adipose cell line from interscapular depot
|
| Organism |
Mus musculus |
| Characteristics |
background strain: 129SVE from Taconic
cell line: BAT6
|
| Treatment protocol |
treated with forskolin (10mM) for 4 hrs.
|
| Growth protocol |
clonal adipose cell line fully differentiated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by TRIzol extraction and purification using QIAGEN Rneasy mini columns according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized at 45C on Affymetrix mouse genome 430 2.0 arrays.
|
| Scan protocol |
The label, hyb and scan were performed by the Dana-Farber Cancer institute Core facility using affymetrix Gene Chip Mouse Genome 430 2.0 arrays according to established methods.
|
| Description |
Gene expression data from clonal adipose cell line of interscapular depot
|
| Data processing |
The data were normalized using the DNA-chip Analyzer(dChip) software.
|
| |
|
| Submission date |
Jul 23, 2012 |
| Last update date |
Jul 24, 2012 |
| Contact name |
jun wu |
| Organization name |
dana-farber cancer institute
|
| Street address |
450 brookline ave. CLSB11117A
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE39562 |
Immortalized clonal brown, beige and white adipose cell lines |
|
