|
| Status |
Public on Mar 01, 2013 |
| Title |
Cells incubated with IL-8 |
| Sample type |
RNA |
| |
|
| Source name |
IS26 (+IL8)
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Chronic lymphocytic leukemia (CLL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip murine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Geneschips were scanned ccording to the manufacturer's instructions.
|
| Data processing |
The data were analyzed using Affymetrix deafault analysis settins and global scaling as normalization method. The trimmed mean tarteg intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 19, 2012 |
| Last update date |
Mar 01, 2013 |
| Contact name |
Idit Shachar |
| E-mail(s) |
idit.shachar@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Street address |
2 Hersel st
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE37430 |
Gene regulation following MIF / IL-8 stimulation |
|
