|
| Status |
Public on Apr 09, 2012 |
| Title |
GBAM1 CD133- indirect co-cultured biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
GBAM1, 10d differentiated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
cell type: GBAM1 CD133-
culture condition: indirect co-cultured
|
| Treatment protocol |
48h of co-culture
|
| Growth protocol |
cells were grown in their respective culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The isolated RNA was amplified using GeneChip 3’ IVT Express Kit (Affymetrix) according to manufacturers instruction
|
| |
|
| Hybridization protocol |
Hybridized to GeneChip Human genome U133A 2.0 array chips (Affymetrix) according to manufacturers instruction
|
| Scan protocol |
according to manufacturers instruction
|
| Description |
Gene expression data from GBAM1 CD133- cell, (differentiated for 10d from GBAM1 CD133+ cells) indirect co-cultured for 48h with astrocytes
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 09, 2012 |
| Last update date |
Apr 09, 2012 |
| Contact name |
Barbara Helen Rath |
| E-mail(s) |
rathbh@mail.nih.gov
|
| Organization name |
National Institute of Health
|
| Department |
National Cancer Institute
|
| Lab |
Radiation Oncology
|
| Street address |
10 Center Drive B3B406
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE37120 |
Expression data from glioblastoma stem-like cells and their differentiated progeny co-cultured with astrocytes |
|
