|
| Status |
Public on Dec 06, 2012 |
| Title |
Osteosarcoma cell line IOR/OS10 |
| Sample type |
RNA |
| |
|
| Source name |
Osteosarcoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IOR/OS10
cell type: Osteosarcoma
sample origin: Primary tumour
gender: female
age (yrs): 10
|
| Biomaterial provider |
Istituto Ortopedico Rizzoli
|
| Growth protocol |
The osteosarcoma cells were grown in RPMI1640 (Lonza, Basel, Switzerland) or DMEM (Lonza) supplemented with 10 % foetal calf serum (PAA Laboratories GmbH, Pasching, Austria), GlutaMAX (Invitrogen, California, USA) and penicillin/streptomycin (Lonza), at 37°C with 5 % CO2. All cells were split when reaching 80 % confluency. The osteoblast cells were maintained in medium provided by the manufacturer, split when reaching 80 % confluency, and harvested when enough cells for DNA and RNA isolation were obtained.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Osteosarcoma cell line IOR/OS10 Illumina HumanWG-6 Expression BeadChip version 2
|
| Data processing |
The data was vst transformed and normalised using quantile normalisation in R
|
| |
|
| Submission date |
Feb 22, 2012 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
leonardm@rr-research.no
|
| Organization name |
The Norwegian Radium Hospital
|
| Department |
Dept. of Tumor Biology
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
N-0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE36001 |
Integrative analysis reveals relationships of genetic and epigenetic alterations in osteosarcoma [gene expression] |
| GSE36004 |
Integrative analysis reveals relationships of genetic and epigenetic alterations in osteosarcoma. |
|
