9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
proband f, past moderate exercise, white blood cells, male, healthy, no medicamentation
