|
| Status |
Public on Jan 21, 2011 |
| Title |
GM12002 cDNA |
| Sample type |
RNA |
| |
|
| Source name |
CEU_LCL
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: lymphoblasts
population: CEU
|
| Biomaterial provider |
http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=GM12002
|
| Treatment protocol |
Two cell lines were treated with 1, 5, and 10uM of 5-azadeoxycytidine every 24 hours for three days. A third cell line was treated with 10uM for 5 and 10 days.
|
| Growth protocol |
The LCLs were grown in T75 flasks in 1X RPMI 1640 Media (invitrogen, Burlington, ON), With 2 mM L-glutamine, 15% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO2. The Fibroblast primary cell lines were grown in medium containing a-MEM (SigmaAldrich) supplemented with 2 mmol/l L-Glutamine, 100 U/mL penicillin, 100 mg/ml streptomycin, and 10% fetal bovine serum (SigmaAldrich) at 37°C with 5% CO2. At 70%-80% confluence, the cells were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from cell lysates resuspended in 600 mL RLT lysis buffer using the Rneasy Mini Kit (Qiagen). DNA was extracted from cell lysates resuspended in 200 mL PBS using the GenElute DNA Miniprep Kit (SigmaAldrich).
|
| Label |
Cy3 and Cy5
|
| Label protocol |
according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
according to manufacturer's instructions
|
| Scan protocol |
according to manufacturer's instructions
|
| Description |
pedigree 1420 daughter
set1
|
| Data processing |
Using GenomeStudio from Illumina
|
| |
|
| Submission date |
Jan 06, 2011 |
| Last update date |
Jan 21, 2011 |
| Contact name |
Tomi Markku Pastinen |
| E-mail(s) |
Tomi.Pastinen@mcgill.ca
|
| Organization name |
McGill University and Génome Québec Innovation Centre
|
| Street address |
740, Dr. Penfield Avenue
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6984 |
| Series (1) |
| GSE26286 |
Genome-wide assessment of imprinted expression in human cells |
|
