|
| Status |
Public on Apr 28, 2010 |
| Title |
ChIP-Seq analysis of H3K4me3 in human cultured CD34 cells; 30E62AAXX090709-7-S |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD34+ cells from cord blood expanded in short term culture; 30E62AAXX090709-7-S
|
| Organism |
Homo sapiens |
| Characteristics |
molecule: genomic DNA
disease: none
biomaterial_provider: FHCRC
biomaterial_type: Primary Cell
cell_type: CD34 Cultured Cells
markers: CD34+
donor_id: cultured CD34_2/20/09
donor_age: NA
donor_health_status: none
donor_sex: Unknown
donor_ethnicity: NA
passage_if_expanded: short term
experiment_type: Histone H3K4me3
extraction_protocol: SDS lysis
extraction_protocol_type_of_sonicator: Bioruptor
extraction_protocol_sonication_cycles: 1
chip_protocol: Bernstein_BROAD_ENCODE_protocol
chip_protocol_chromatin_amount: 10 million cells
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_protocol_bead_amount: 70 ul bed volume
chip_protocol_antibody_amount: 2-15 ug
chip_antibody: H3K4Me3
chip_antibody_provider: Millipore
chip_antibody_catalog: 07-473
chip_antibody_lot: R0903B0054
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
sample_term_id: CL_0001059
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: Solexa-12900
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.2239
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.2238
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM537629_BI.CD34_Cultured_Cells.H3K4me3.cultured_CD34_2_20_09.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: 30E62AAXX090709-7-S.hg19.level.1
ANALYSIS TITLE: Mapping of CD34 Cultured Cells Histone H3K4me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H3K4me3 ChIP-Seq on CD34 Cultured Cells, Donor cultured CD34_2/20/09 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4696
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 2
QUALITY SCORES:
NUMBER_OF_H3K4me3_EXPERIMENTS_SCORED: 62
FINDPEAKS_SCORE: 0.5108
FINDPEAKS_PERCENTILE: 74
HOTSPOT_SCORE: 0.4645
HOTSPOT_PERCENTILE: 64
IROC_SCORE: 0.9885
IROC_PERCENTILE: 5
POISSON_SCORE: 0.5793
POISSON_PERCENTILE: 73
**********************************************************************
ANALYSIS FILE NAME: GSM537629_BI.CD34_Cultured_Cells.H3K4me3.cultured_CD34_2_20_09.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: 30E62AAXX090709-7-S.hg19.level.2
ANALYSIS TITLE: Raw Signal Density Graphs of CD34 Cultured Cells Histone H3K4me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H3K4me3 ChIP-Seq read mappings from CD34 Cultured Cells, Donor cultured CD34_2/20/09 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4970
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 2
BROWSER_TRACK_NAME: CD34 H3K4me3 20
BROWSER_TRACK_DESCRIPTION: BI CD34 Cultured Cells Histone H3K4me3 Donor cultured CD34_2/20/09 EA Release 2
QUALITY SCORES:
NUMBER_OF_H3K4me3_EXPERIMENTS_SCORED: 62
FINDPEAKS_SCORE: 0.5108
FINDPEAKS_PERCENTILE: 74
HOTSPOT_SCORE: 0.4645
HOTSPOT_PERCENTILE: 64
IROC_SCORE: 0.9885
IROC_PERCENTILE: 5
POISSON_SCORE: 0.5793
POISSON_PERCENTILE: 73
**********************************************************************
|
| |
|
| Submission date |
Apr 26, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE19465 |
BI Human Reference Epigenome Mapping Project: ChIP-Seq in human subject |
|
| Relations |
| BioSample |
SAMN00012087 |
| SRA |
SRX019740 |
| Named Annotation |
GSM537629_BI.CD34_Cultured_Cells.H3K4me3.cultured_CD34_2_20_09.wig.gz |
