|
| Status |
Public on Sep 23, 2011 |
| Title |
S0053 |
| Sample type |
RNA |
| |
|
| Source name |
Blood from Control
|
| Organism |
Homo sapiens |
| Characteristics |
case status: Control
Stage: --
morphology: --
smoking status: Former
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and following clean-up with Qiagen Rneasy Mini Kit were performed according to the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 to 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
10 ug fragmented cRNA were hybridized for 16 hr at 45C on GeneChip H133-A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner.
|
| Description |
Control
|
| Data processing |
Gene expression data were processed and normalized using Bioconductor Affy package, based on the Robust Multichip Average (RMA) method5 for single-channel Affymetrix chips
|
| |
|
| Submission date |
Feb 04, 2010 |
| Last update date |
Sep 23, 2011 |
| Contact name |
Melissa Rotunno |
| E-mail(s) |
rotunnom@mail.nih.gov
|
| Phone |
301-402-1622
|
| Fax |
301-402-4489
|
| Organization name |
NIH/NCI
|
| Department |
DCEG
|
| Lab |
GEB
|
| Street address |
6120 Executive Blvd
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE20189 |
A gene expression signature from peripheral whole blood for stage I lung adenocarcinoma |
|
