|
| Status |
Public on Dec 19, 2009 |
| Title |
K562 STI sample 6 |
| Sample type |
RNA |
| |
|
| Source name |
K562 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: STI (Imatinib)
cell line: K562
|
| Treatment protocol |
K562 cells were treated with 0.5 µM Imatinib (STI571), 0.05 µM Nilotinib (AMN107) (IC50 concentration) or DMSO as control for 24 hours.
|
| Growth protocol |
RPMI, 10% FBS, 2 mM L-Gluthamine, 100 U/ml Penicillin, 100 µg/ml Streptomycin, 37°C humified incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kit (Qiagen; 74106)
|
| Label |
biotin
|
| Label protocol |
Biotin Label Protocol GeneChip Expression 3’-Amplification One-Cycle-Target, 10 ug cRNA were used.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG U133 A2.0. GeneChips were washed and stained in the Gene Chips® Fluidics Station; Affymetrix.
|
| Scan protocol |
GeneChips were scanned using the GenArray® 2500 scanner (Agilent).
|
| Description |
K562 cells (CML cell line) treated with IC50 concentration of STI571 (Imatinib) (0.5 µM) for 24 hours
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Scaling factor was 500.
|
| |
|
| Submission date |
Dec 18, 2009 |
| Last update date |
Dec 18, 2009 |
| Contact name |
Daniela Bruennert |
| E-mail(s) |
Daniela.Bruennert@med.uni-duesseldorf.de
|
| Phone |
0211/8114869
|
| Organization name |
University Clinic Duesseldorf
|
| Department |
Clinic for Hematology, Oncology and clin. Immunology
|
| Lab |
Haematologisches Forschungslabor
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE19567 |
Imatinib and Nilotinib induced molecular changes in Philadelphia chromosome-positive Chronic Myelogenous Leukemia |
|
