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Status |
Public on Dec 19, 2009 |
Title |
K562 AMN107 sample 4 |
Sample type |
RNA |
|
|
Source name |
K562 cell line
|
Organism |
Homo sapiens |
Characteristics |
treatment: AMN107 (Nilotinib) cell line: K562
|
Treatment protocol |
K562 cells were treated with 0.5 µM Imatinib (STI571), 0.05 µM Nilotinib (AMN107) (IC50 concentration) or DMSO as control for 24 hours.
|
Growth protocol |
RPMI, 10% FBS, 2 mM L-Gluthamine, 100 U/ml Penicillin, 100 µg/ml Streptomycin, 37°C humified incubator
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy mini kit (Qiagen; 74106)
|
Label |
biotin
|
Label protocol |
Biotin Label Protocol GeneChip Expression 3’-Amplification One-Cycle-Target, 10 ug cRNA were used.
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG U133 A2.0. GeneChips were washed and stained in the Gene Chips® Fluidics Station; Affymetrix.
|
Scan protocol |
GeneChips were scanned using the GenArray® 2500 scanner (Agilent).
|
Description |
K562 cells (CML cell line) treated with IC50 concentration of AMN107 (Nilotinib) (0.05 µM) for 24 hours
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Scaling factor was 500.
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|
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Submission date |
Dec 18, 2009 |
Last update date |
Dec 18, 2009 |
Contact name |
Daniela Bruennert |
E-mail(s) |
Daniela.Bruennert@med.uni-duesseldorf.de
|
Phone |
0211/8114869
|
Organization name |
University Clinic Duesseldorf
|
Department |
Clinic for Hematology, Oncology and clin. Immunology
|
Lab |
Haematologisches Forschungslabor
|
Street address |
Universitaetsstr. 1
|
City |
Duesseldorf |
ZIP/Postal code |
40225 |
Country |
Germany |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE19567 |
Imatinib and Nilotinib induced molecular changes in Philadelphia chromosome-positive Chronic Myelogenous Leukemia |
|