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Status
Public on Oct 27, 2005
Title
GM12003_rep1
Sample type
RNA
Source name
Lymphoblastoid cell line
Organism
Homo sapiens Characteristics
Coriell cell line repository identifier: GM12003http://locus.umdnj.edu/nigms/nigms_cgi/display.cgi?GM12003
Biomaterial provider
Coriell Cell Repositories
Extracted molecule
total RNA
Description
Sample description = RNA was extracted from lymphoblastoid cells of the CEPH individuals from the International HapMap Project. The cells were grown at a density of 5 x 105 cells/ ml in RPMI 1640 with 15% FBS, 1% penicillin-streptomycin, 2mM L-glutamine at 37°C in a humidified 5% CO2 chamber. RNA samples were processed and hybridized onto the Affymetrix Human Focus Arrays according to the manufacturer’s suggestion. Arrays were scanned and analyzed using MAS 5.0 software (Affymetrix). Expression intensity was sclaed to 500 and log2 transformed.
Submission date
Apr 20, 2005
Last update date
Nov 30, 2005
Contact name
Michael Patrick Morley
E-mail(s)
mmorley@pennmedicine.upenn.edu Phone
215-898-2026
Organization name
Perelman School of Medicine at the University of Pennsylvania
Department
Penn Cardiovascular Institute
Street address
3400 Civic Center Blvd, Bldg 421
City
Philadelphia
State/province
PA
ZIP/Postal code
19104
Country
USA
Platform ID
GPL201 Series (2)
GSE2552 Mapping Determinants of Human Gene Expression by Regional and Whole Genome Association
GSE5859 Allelic Differences Account for Gene Expression Differences Among Population.
Data table header descriptions ID_REF
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 1007_s_at
248.4
P
1053_at
385.1
P
117_at
14.8
A
121_at
618.2
P
1255_g_at
9.6
A
1294_at
452.7
P
1316_at
48.7
A
1320_at
7.3
A
1431_at
5.1
A
1438_at
77.3
A
1487_at
350.9
P
1494_f_at
120.1
A
1598_g_at
131.4
A
160020_at
110.6
M
1729_at
537.9
P
1773_at
91.7
A
177_at
52.7
P
1861_at
137.1
A
200000_s_at
1652.4
P
200001_at
3556.3
P
8793 156 Kbytes .Supplementary file Size Download File type/resource GSM48655.CEL.gz
1.2 Mb
(ftp) (http) CEL
