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Status
Public on Jun 22, 2012
Title
RIS7_swap
Sample type
RNA
Channel 1 Source name
Reference
Organism
Homo sapiens Characteristics
sample type: pool of 15 human cell lines
Biomaterial provider
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
Extracted molecule
total RNA
Extraction protocol
Trizol
Label
Alexa Fluor succinimidyl ester 555
Label protocol
Protocol Cy3/Cy5
Channel 2 Source name
RIS7
Organism
Homo sapiens Characteristics
gender: male, disease state: schizophrenia, agent: risperidone
Extracted molecule
total RNA
Extraction protocol
kit Pico Pure RNA Isolation Kit
Label
Alexa Fluor succinimidyl ester 647
Label protocol
Protocol Cy3/Cy5
Hybridization protocol
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Scan protocol
Hardware: Perkinelmer / Software: ScanArray
Description
A pool of RNAs obtained from 15 distinct human cell lines (Daudi from Burkitt's lymphoma, DLD-1 from colorectal adenocarcinoma, DU 145 from prostate carcinoma, FaDu from pharinx squamous cell carcinoma, GM-637 from fibroblast, H-146 from small cell lung carcinoma, HT-1080 from fibrosarcoma, HB4a from normal mammary luminal epithelium, HEK from human embryonic kidney, Jurkat from acute T cell leukemia, Saos-2 from osteosarcoma, SK-BR-3 from breast adenocarcinoma, SK-MEL-28 from melanoma, T24 from bladder transitional cell carcinoma and T98G from glioblastoma multiforme) were used as reference. Total RNA was amplified following the same protocol and used as reference for microarray hybridizations.
Data processing
Lowesss
Submission date
Nov 17, 2009
Last update date
Jun 22, 2012
Contact name
Renato David Puga
Organization name
Universidade de São Paulo
Department
Instituto de Psiquiatria
Lab
Laboratório de Genética
Street address
Dr Ovidio Pires de Campos,785
City
São Paulo
State/province
São Paulo
ZIP/Postal code
05403-010
Country
Brazil
Platform ID
GPL9692 Series (1)
GSE19112 Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia
Data table header descriptions ID_REF IntCH1 Channel 1 Intensity
BkgCH1 BackGround Channel 1
IntCH2 Channel 2 Intensity
BkgCH2 BackGround Channel 2
VALUE Lowess-normalized signal intensity, log2 test/reference
Data table ID_REF IntCH1 BkgCH1 IntCH2 BkgCH2 VALUE 1
1549
174
5271
85
null
2
432
152
90
84
null
3
537
169
135
83
0.0281298
4
547
169
153
82
-0.013892
5
576
174
146
84
0.195014
6
1721
191
1156
84
-0.65351
7
472
177
93
84
null
8
414
147
97
82
null
9
418
149
92
82
null
10
410
147
90
84
null
11
853
164
824
83
-1.27701
12
1711
175
718
83
0.104878
13
632
181
176
84
0.23331
14
477
178
91
83
null
15
442
154
96
84
-0.374362
16
3589
173
320
82
2.62875
17
532
164
242
83
-0.574073
18
618
164
584
84
null
19
638
170
311
85
-0.402454
20
779
180
244
85
0.401183
2352 63 Kbytes .Supplementary data files not provided Processed data included within Sample table
