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Status
Public on Jun 22, 2012
Title
RIS5_swap
Sample type
RNA
Channel 1 Source name
Reference
Organism
Homo sapiens Characteristics
sample type: pool of 15 human cell lines
Biomaterial provider
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
Extracted molecule
total RNA
Extraction protocol
Trizol
Label
Alexa Fluor succinimidyl ester 555
Label protocol
Protocol Cy3/Cy5
Channel 2 Source name
RIS5
Organism
Homo sapiens Characteristics
gender: male, disease state: schizophrenia, agent: risperidone
Extracted molecule
total RNA
Extraction protocol
kit Pico Pure RNA Isolation Kit
Label
Alexa Fluor succinimidyl ester 647
Label protocol
Protocol Cy3/Cy5
Hybridization protocol
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Scan protocol
Hardware: Perkinelmer / Software: ScanArray
Description
A pool of RNAs obtained from 15 distinct human cell lines (Daudi from Burkitt's lymphoma, DLD-1 from colorectal adenocarcinoma, DU 145 from prostate carcinoma, FaDu from pharinx squamous cell carcinoma, GM-637 from fibroblast, H-146 from small cell lung carcinoma, HT-1080 from fibrosarcoma, HB4a from normal mammary luminal epithelium, HEK from human embryonic kidney, Jurkat from acute T cell leukemia, Saos-2 from osteosarcoma, SK-BR-3 from breast adenocarcinoma, SK-MEL-28 from melanoma, T24 from bladder transitional cell carcinoma and T98G from glioblastoma multiforme) were used as reference. Total RNA was amplified following the same protocol and used as reference for microarray hybridizations.
Data processing
Lowesss
Submission date
Nov 17, 2009
Last update date
Jun 22, 2012
Contact name
Renato David Puga
Organization name
Universidade de São Paulo
Department
Instituto de Psiquiatria
Lab
Laboratório de Genética
Street address
Dr Ovidio Pires de Campos,785
City
São Paulo
State/province
São Paulo
ZIP/Postal code
05403-010
Country
Brazil
Platform ID
GPL9692 Series (1)
GSE19112 Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia
Data table header descriptions ID_REF IntCH1 Channel 1 Intensity
BkgCH1 BackGround Channel 1
IntCH2 Channel 2 Intensity
BkgCH2 BackGround Channel 2
VALUE Lowess-normalized signal intensity, log2 test/reference
Data table ID_REF IntCH1 BkgCH1 IntCH2 BkgCH2 VALUE 1
8459
169
11803
86
null
2
397
141
91
81
null
3
792
163
466
86
-0.41257
4
1351
217
311
86
1.19615
5
1205
474
245
92
0.85279
6
3994
262
5536
90
-1.53508
7
414
143
90
83
null
8
402
138
95
83
null
9
399
139
92
82
null
10
426
150
97
84
null
11
1588
200
790
82
-0.162326
12
3145
392
1787
87
-0.548277
13
1262
240
382
88
0.681847
14
425
147
90
81
null
15
531
160
171
85
-0.262745
16
7222
178
594
84
2.54744
17
725
168
375
86
-0.279208
18
821
173
422
85
null
19
1218
205
516
85
0.149152
20
1553
189
403
85
1.01672
2352 65 Kbytes .Supplementary data files not provided Processed data included within Sample table
