NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM471882 Query DataSets for GSM471882
Status Public on Jun 22, 2012
Title HAL6_swap
Sample type RNA
 
Channel 1
Source name Reference
Organism Homo sapiens
Characteristics sample type: pool of 15 human cell lines
Biomaterial provider Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
Extracted molecule total RNA
Extraction protocol Trizol
Label Alexa Fluor succinimidyl ester 555
Label protocol Protocol Cy3/Cy5
 
Channel 2
Source name HAL6
Organism Homo sapiens
Characteristics gender: male, disease state: schizophrenia, agent: haloperidol
Extracted molecule total RNA
Extraction protocol kit Pico Pure RNA Isolation Kit
Label Alexa Fluor succinimidyl ester 647
Label protocol Protocol Cy3/Cy5
 
 
Hybridization protocol Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Washing - Round 1 - Solution: SSC 2X / Temperature:42 / Time:10 / Washing Number: 1
Washing - Round 2 - Solution: SSC 0.1X, SDS 0.1% / Temperature:42 / Time:10 / Washing Number: 2
Washing - Round 3 - Solution: SSC 0.1X / Temperature:42 / Time:10 / Washing Number: 2
Scan protocol Hardware: Perkinelmer / Software: ScanArray
Description A pool of RNAs obtained from 15 distinct human cell lines (Daudi from Burkitt's lymphoma, DLD-1 from colorectal adenocarcinoma, DU 145 from prostate carcinoma, FaDu from pharinx squamous cell carcinoma, GM-637 from fibroblast, H-146 from small cell lung carcinoma, HT-1080 from fibrosarcoma, HB4a from normal mammary luminal epithelium, HEK from human embryonic kidney, Jurkat from acute T cell leukemia, Saos-2 from osteosarcoma, SK-BR-3 from breast adenocarcinoma, SK-MEL-28 from melanoma, T24 from bladder transitional cell carcinoma and T98G from glioblastoma multiforme) were used as reference. Total RNA was amplified following the same protocol and used as reference for microarray hybridizations.
Data processing Lowesss
 
Submission date Nov 17, 2009
Last update date Jun 22, 2012
Contact name Renato David Puga
Organization name Universidade de São Paulo
Department Instituto de Psiquiatria
Lab Laboratório de Genética
Street address Dr Ovidio Pires de Campos,785
City São Paulo
State/province São Paulo
ZIP/Postal code 05403-010
Country Brazil
 
Platform ID GPL9692
Series (1)
GSE19112 Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia

Data table header descriptions
ID_REF
IntCH1 Channel 1 Intensity
BkgCH1 BackGround Channel 1
IntCH2 Channel 2 Intensity
BkgCH2 BackGround Channel 2
VALUE Lowess-normalized signal intensity, log2 test/reference

Data table
ID_REF IntCH1 BkgCH1 IntCH2 BkgCH2 VALUE
1 1616 83 1857 81 null
2 213 79 94 75 null
3 248 81 93 76 null
4 232 79 95 77 null
5 238 81 94 77 null
6 350 88 316 83 -0.554096
7 253 84 103 72 null
8 221 77 102 76 null
9 216 76 93 75 null
10 213 76 91 74 null
11 237 80 94 79 null
12 325 83 98 79 0.0505738
13 267 85 104 82 null
14 251 83 93 82 null
15 224 79 98 76 null
16 1700 85 91 81 5.60894
17 282 81 135 79 -0.465977
18 241 80 97 78 null
19 243 80 98 77 null
20 267 85 106 79 null

Total number of rows: 2352

Table truncated, full table size 56 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap