GEO help: Mouse over screen elements for information.
Status
Public on Jun 22, 2012
Title
CON3_swap
Sample type
RNA
Channel 1 Source name
Reference
Organism
Homo sapiens Characteristics
sample type: pool of 15 human cell lines
Biomaterial provider
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
Extracted molecule
total RNA
Extraction protocol
Trizol
Label
Alexa Fluor succinimidyl ester 555
Label protocol
Protocol Cy3/Cy5
Channel 2 Source name
CON3
Organism
Homo sapiens Characteristics
gender: male, disease state: control
Extracted molecule
total RNA
Extraction protocol
kit Pico Pure RNA Isolation Kit
Label
Alexa Fluor succinimidyl ester 647
Label protocol
Protocol Cy3/Cy5
Hybridization protocol
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Scan protocol
Hardware: Perkinelmer / Software: ScanArray
Description
A pool of RNAs obtained from 15 distinct human cell lines (Daudi from Burkitt's lymphoma, DLD-1 from colorectal adenocarcinoma, DU 145 from prostate carcinoma, FaDu from pharinx squamous cell carcinoma, GM-637 from fibroblast, H-146 from small cell lung carcinoma, HT-1080 from fibrosarcoma, HB4a from normal mammary luminal epithelium, HEK from human embryonic kidney, Jurkat from acute T cell leukemia, Saos-2 from osteosarcoma, SK-BR-3 from breast adenocarcinoma, SK-MEL-28 from melanoma, T24 from bladder transitional cell carcinoma and T98G from glioblastoma multiforme) were used as reference. Total RNA was amplified following the same protocol and used as reference for microarray hybridizations.
Data processing
Lowesss
Submission date
Nov 17, 2009
Last update date
Jun 22, 2012
Contact name
Renato David Puga
Organization name
Universidade de São Paulo
Department
Instituto de Psiquiatria
Lab
Laboratório de Genética
Street address
Dr Ovidio Pires de Campos,785
City
São Paulo
State/province
São Paulo
ZIP/Postal code
05403-010
Country
Brazil
Platform ID
GPL9692 Series (1)
GSE19112 Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia
Data table header descriptions ID_REF IntCH1 Channel 1 Intensity
BkgCH1 BackGround Channel 1
IntCH2 Channel 2 Intensity
BkgCH2 BackGround Channel 2
VALUE Lowess-normalized signal intensity, log2 test/reference
Data table ID_REF IntCH1 BkgCH1 IntCH2 BkgCH2 VALUE 1
1137
97
1073
84
null
2
258
85
89
81
null
3
273
86
95
81
null
4
280
86
130
83
-0.644817
5
282
87
100
80
-0.497807
6
531
93
862
83
-1.39903
7
261
87
91
80
null
8
265
79
92
81
null
9
262
85
90
80
null
10
255
84
92
78
null
11
285
88
176
81
-0.788773
12
727
91
293
82
1.14052
13
308
89
138
82
-0.376559
14
263
85
94
81
null
15
295
89
93
82
null
16
1551
92
115
82
4.49676
17
308
88
106
83
-0.188249
18
296
87
292
82
null
19
309
88
177
83
-0.480702
20
341
91
155
82
-0.0963163
2352 60 Kbytes .Supplementary data files not provided Processed data included within Sample table
