|
| Status |
Public on Jun 22, 2012 |
| Title |
CLO9_control |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
CLO9
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male, disease state: schizophrenia, agent: clozapine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit Pico Pure RNA Isolation Kit
|
| Label |
Alexa Fluor succinimidyl ester 555
|
| Label protocol |
Protocol Cy3/Cy5
|
| |
|
| Channel 2 |
| Source name |
Reference
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pool of 15 human cell lines
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00637
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Alexa Fluor succinimidyl ester 647
|
| Label protocol |
Protocol Cy3/Cy5
|
| |
|
| |
| Hybridization protocol |
Hibridization - Solution: 5 X SSC; 0,1% SDS; 4 ug poly A DNA; 4 ug Cot1 DNA; 5X Denhardts ; 10 ug Esperma de salmao, Formamida 25%. / Temperature:14 a 24 hs / Time:42 C / Touch Down:
Washing - Round 1 - Solution: SSC 2X / Temperature:42 / Time:10 / Washing Number: 1
Washing - Round 2 - Solution: SSC 0.1X, SDS 0.1% / Temperature:42 / Time:10 / Washing Number: 2
Washing - Round 3 - Solution: SSC 0.1X / Temperature:42 / Time:10 / Washing Number: 2
|
| Scan protocol |
Hardware: Perkinelmer / Software: ScanArray
|
| Description |
Blood samples from 9 male patients, aging between 18-65 years, diagnosed with schizophrenia and treated with Clozapine were used for total RNA extraction. Study protocol was approved by the Ethics Committee of the Hospital, and performed in accordance with the Declaration of Helsinki. A two-round linear amplification procedure based on T7-driven amplification was carried out. Amplified RNA was then used in a transcriptase reverse reaction into cDNA in the presence of Alexa 555 or Alexa 647. Dye swap was performed for each sample analyzed and used as replicate samples.
|
| Data processing |
Lowesss
|
| |
|
| Submission date |
Nov 17, 2009 |
| Last update date |
Jun 22, 2012 |
| Contact name |
Renato David Puga |
| Organization name |
Universidade de São Paulo
|
| Department |
Instituto de Psiquiatria
|
| Lab |
Laboratório de Genética
|
| Street address |
Dr Ovidio Pires de Campos,785
|
| City |
São Paulo |
| State/province |
São Paulo |
| ZIP/Postal code |
05403-010 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL9692 |
| Series (1) |
| GSE19112 |
Using the expression profile of peripheral blood to investigate regulatory pathways in schizophrenia |
|
