|
| Status |
Public on Jul 28, 2009 |
| Title |
prostate cancer epithelial cells from EA men RC88T |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer epithelial cells from European-American men
|
| Organism |
Homo sapiens |
| Characteristics |
tumor stage: Tx
gleason's sum: 6
seminal vesicle invasion: neg
lymph node invasion: Unk
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Cells were grown to the same confluence (about 80%) in a K-SFM supplemented with bovine pituitary extract and recombinant epidermal growth factor (Life Technologies, Inc., Gaithersburg, MD).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
RNA labeling was performed according to Affymetrix standard protocol for one-cycle target labeling method.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized in triplicates to Affymetrix GeneChip HG-U133A 2.0 arrays (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G
|
| Description |
gene expression data for prostate cancer epithelial cells from EA men
|
| Data processing |
We applied RMA for background adjustment, quantile method for normalization, and the “median polish” for summarization. The triplicate arrays representing the same subject were averaged. The random variance model implemented in BRB-ArrayTools (NCI, Bethesda, MD) was used for this analysis
|
| |
|
| Submission date |
Jul 27, 2009 |
| Last update date |
Jul 27, 2009 |
| Contact name |
Olga A Timofeeva |
| E-mail(s) |
oat@georgetown.edu
|
| Phone |
202-687-8810
|
| Fax |
202-687-2221
|
| Organization name |
Georgetown University Medical Center
|
| Department |
Oncology
|
| Street address |
3900 Reservoir Rd NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE17356 |
Expression data from prostate cancer epithelial cells from African American and European American men |
|
