|
| Status |
Public on Jul 13, 2010 |
| Title |
MCF7_hypoxia_18h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7_hypoxia_18h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: breast cancer
growth condition: hypoxia
shrna: non-specific
|
| Treatment protocol |
Cells were transfected with control shRNA or Reptin shRNA and grown under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions for 18 hours.
|
| Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Refer to the Illumina Gene Expression System Manual.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Illumina BeadScan 500 System.
|
| Description |
MCF7 cells transfected with non-specific shRNA and grown under Hypoxic conditions.
|
| Data processing |
The data were normalised using the quantile normalization method with beadarray version 1.10 and lumi version 1.8.3 in R/Bioconductor package.
|
| |
|
| Submission date |
Apr 08, 2009 |
| Last update date |
Jul 13, 2010 |
| Contact name |
Minhyung Kim |
| E-mail(s) |
Minhyung.Kim@cshs.org
|
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Urology
|
| Street address |
8700 Beverly Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE15530 |
Genome-wide analysis of gene expression perturbed by reptin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
|
